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  • Assays for the Evaluation of HIV-1 Integrase Inhibitors

    Assays for the Evaluation of HIV-1 Integrase Inhibitors

    Integration is an essential step in the replication cycle of a retrovirus, such

    2024-12-27 6
  • 细胞增殖的检验方法:BrdU标记法

    细胞增殖的检验方法:BrdU标记法

    BrdU 标记法 1. 细胞以 1.5 105 /ml 细胞数接种于直径 35ml 培养皿中(内放置一盖玻片),培养 1 天,用含 0.4 % FCS 培养液

    2024-12-27 6
  • 细胞冻存方法

    细胞冻存方法

    1.细胞:选对数生长期细胞,收集细胞24小时前换液一次。2.计数:按常规方法把细胞制成细胞悬液,计数,令细胞密度达5106/ml,离心去上清,吸入离心管中。3.

    2024-12-27 5
  • Cryopreservation of Semen from Domestic Livestock

    Cryopreservation of Semen from Domestic Livestock

    Although the first observations concerning low temperature preservation of sperm

    2024-12-27 4
  • Trypsinizing cells

    Trypsinizing cells

    There are many procedures with which to trypsinize cells. All include washing th

    2024-12-27 5
  • Flow Cytometric Characterization of Neural Precursor Cells and Their Progeny

    Flow Cytometric Characterization of Neural Precursor Cells and Their Progeny

    It is now clear that the adult central nervous system contains a population of n

    2024-12-27 6
  • Electron Cryomicroscopy of Fibrillar Collagens

    Electron Cryomicroscopy of Fibrillar Collagens

    Collagen fibrils in tissue are generally heterotypic with more than one type of

    2024-12-27 4
  • Breast Cancer Cell Movement: Imaging Invadopodia by TIRF and IRM Microscopy

    Breast Cancer Cell Movement: Imaging Invadopodia by TIRF and IRM Microscopy

    Invadopodia are hair-like membrane protrusions projecting from the ventral side

    2024-12-27 5
  • Activity-Based Profiling of Lipases in Living Cells

    Activity-Based Profiling of Lipases in Living Cells

    The ultimate goal of proteomics is to characterize the function of all proteins

    2024-12-27 4
  • Generation of Differentially Polyglutamylated Microtubules

    Generation of Differentially Polyglutamylated Microtubules

    Microtubules are cytoskeletal structures built of alpha- and beta-tubulins. Alth

    2024-12-27 3
  • Suppression of Pancreatic and Colon Cancer Cells by Antisense K-ras RNA Expression VectorsM. Ian Phi

    Suppression of Pancreatic and Colon Cancer Cells by Antisense K-ras RNA Expression VectorsM. Ian Phi

    Various genetic abnormalities accumulate during multistage carcinogenesis. The c

    2024-12-27 9
  • Culture Systems: Low-Oxygen Culture

    Culture Systems: Low-Oxygen Culture

    The tension of oxygen measured in the oviducts of several mammals was 5–8.7 %, b

    2024-12-27 7
  • Peptidomics: Divide et Impera

    Peptidomics: Divide et Impera

    The term “peptidomics” can be defined as the systematic analysis of the peptide

    2024-12-27 8
  • Visualizing Receptor Endocytosis and Trafficking

    Visualizing Receptor Endocytosis and Trafficking

    G-protein-coupled 7 transmembrane domain receptors (GPCR-7TMR) represent the lar

    2024-12-27 9
  • ALDH as a Marker for Enriching Tumorigenic Human Colonic Stem Cells

    ALDH as a Marker for Enriching Tumorigenic Human Colonic Stem Cells

    Aldehyde dehydrogenase (ALDH) can be used as a marker to isolate, propagate, and

    2024-12-27 9