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CHO细胞表达系统在疫苗研制中的应用
分子生物学、分子免疫学等学科的发展使基因工程疫苗具有越来越重要的地位。在基因工程疫苗研究的动物细胞表达系统中,最具代表性的就是中国仓鼠卵巢细胞(Chinese
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Southern Blotting of Genomic DNA from Lung and Its Tumors: Application to Analysis of Allele Loss on
Lung cancer is one of the most common cancers afflicting the citizens of develop
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RT-PCR Quantitation of HSP60 mRNA Expression
Heat shock protein 60 (HSP60, HSPD1) is a “chaperonin” that facilitates folding
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Selective Immortalization of Tumor-Specific T Cells to Establish Long-Term T-Cell Lines Maintaining
Antigen-specific T cells play a key role in cellular immune response against can
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Phosphatidylinositol Kinase Activity in Ovarian Cancer Cells
Phosphoinositides constitute less than 0.1% of total cellular lipids, yet accumu
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华人博士发明单个神经细胞及突触体内标识技术
斯坦福大学生物学系神经生物学博士李凌,将在近期的《PLoS ONE》杂志上发表文章,宣布发明了一种对单个神经元细胞及突触进行体内标识的技术,并利用此技术成功地
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Association of Nonreceptor Tyrosine Kinase c-Yes with Tight Junction Protein Occludin by Coimmunopre
Immunoprecipitation is one of the most commonly used techniques to study protein
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FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas
Fluorescence In Situ Hybridization (FISH) is a powerful and robust technique all
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Production of Ligand-Specific Mutants Using a Yeast Two-Hybrid Mating Assay
Acquiring functional knowledge from protein-protein interaction studies often ne
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Measurement of Multiple Drug Resistance Transporter Activity in Putative Cancer Stem/Progenitor Cell
Multiple drug resistance, mediated by the expression and activity of ABC-transpo
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Simultaneous Humanization and Affinity Optimization of Monoclonal Antibodies
Monoclonal antibodies (MAbs) have broad therapeutic applications in cancer thera
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Control of Mitotic Entry After DNA Damage in Drosophila
In the presence of DNA damage, cells delay the entry into mitosis, presumably to
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Apoptotic Caspase Activation and Activity
Caspases are central to the execution of apoptosis. Their proteolytic activity i
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DNA-Mediated Gene Transfer
Transfection is the process by which exogenous DNA is transferred into eukaryoti
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Analyzing the Spindle Checkpoint in Yeast and Frogs
The spindle checkpoint is an evolutionarily conserved regulatory mechanism that