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Purification of Growth Factor mRNA in Renal Tissues:bFGF-2, FGF-2, TGFα, and EGFR
Growth factors are polypeptides that induce cell mitogenicity, and thus play an
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Flow Cytometric Analysis of p53-Induced Apoptosis
The p53 protein is a key player in the cellular response to stress conditions. A
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Microdissection of Histologic Sections: Manual and Laser Capture Microdissection Techniques
The molecular analysis of human cancer is complicated by the difficulty in obtai
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Isolation of Human Fab Fragments Against Ovarian Carcinoma Using Guided Selection
Ovarian cancer remains a major health problem in the United States and most West
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干细胞和衰老的终结（第四部分）
“生命潜能：干细胞、克隆和再生”之Stem Cells and the End of Aging主持人：Thomas R.Cech, Ph.D.Presiden
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外源基因在真核细胞表达技术
本篇中主要介绍生化方法及物理方法（电穿孔法）一、生化方法1、磷酸钙介导的质粒DNA转染真核细胞（1）转染前24 h，通过胰酶消化收集细胞，用适当的完全培养基以1
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哺乳动物细胞表达系统及其研究进展
按照宿主细胞的类型，可将基因表达系统大致分为原核、酵母、植物、昆虫和哺乳动物细胞表达系统。与其它系统相比，哺乳动物细胞表达系统的优势在于能够指导蛋白质的正确折叠
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Direct tissue explant method for derivation and study of extravillous trophoblas
From early post-implantation stages to approximately 18 weeks' gestation, cy
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Real-Time Polymerase Chain Reaction Quantitation of Relative Expression of Genes Modulated by p53 Us
Real-time quantitative polymerase chain reaction (QPCR) using the Roche LightCyc
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G418原代细胞的筛选
zhaolifei认为：分析转化的功能和表达需要DNA稳定转染至宿主细胞染色体。外源基因进入细胞后，部分能够通过细胞质进入细胞核内，根据细胞类型，至多80%的进
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xCELLigence系统内源性GPCRs细胞功能研究
xCELLigence实时细胞分析系统主要应用受体活性 特征性研究xCELLigence 系统内源性GPCRs 细胞功能图作者 Jeff Irelan （左）
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xCELLigence系统实时检测神经毒性
xCELLigence实时细胞分析系统主要应用（一）：神经毒性 特征性研究： xCELLigence系统实时检测神经细胞死亡关键词：神经细胞死亡、xCELLi
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Calcium Phosphate Transfection of PC12 Cells 磷酸钙法转染PC12细胞
Steven Finkbeiner, Departments of Neurology and Physiology, UCSFProtocol note: h
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Evaluating Response to Antineoplastic Drug Combinations in Tissue Culture Models
The mainstay of clinical antineoplastic chemotherapy is multiagent combinations,
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原代上皮细胞与原代成纤维细胞培养的分离纯化
PriCells-原代上皮细胞与原代成纤维细胞培养的分离纯化原代细胞、传代细胞绝大多数都呈混合生长，既有上皮样细胞又有纤维样细胞，纤维样细胞又包括成纤维细胞、肌
