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用LIPO2000转染HepG2细胞的问题
乡乡问:最近本人在做HepG2细胞的转染,转染的是GFP质粒,按照LIPO2000的说明书使用后,发现细胞的死亡很严重,24h时差不多死了一半。而且我在转染前细
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腺病毒能在293中复制吗?
腺病毒可以在293A细胞中进行转录,表达并可进行包装复制出大量高滴度病毒。293T是一种逆转录包装表达细胞,我做过也可进行表达,但还没有具体鉴定表达后的病毒是否
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Cytogenetics in Myelodysplastic Syndromes
Cytogenetic information in patients with myelodysplastic syndrome (MDS) is impor
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Detection and Quantification of Leukemia-Specific Rearrangements
A number of leukemia-specific chromosomal translocations have been identified th
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细胞因子的检测方法
细胞因子(cytokine)是由细胞分泌的具有生物活性的小分子蛋白物质的统称。在免疫应答过程中,细胞因子在免疫调节、炎症应答、肿瘤转移等生理和病理过程中起重要作
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Idiotype Gene Rescue in Follicular Lymphoma
Beyond the morphological, immunophenotypic, and genetic information used for the
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Clinical Protocol for p53 Gene Therapy for Liver Tumors
This chapter is intended to help other workers with the preparation of human gen
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Tailoring Natural Effector Functions: Antibody Engineering Beyond Humanization
A large number of new biological drugs in clinical development from the biotechn
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抑制大肠癌向肝脏转移的新方法
日本研究人员在新一期美国《国家科学院学报》网络版上报告说,通过阻止骨髓细胞内的某种蛋白质与激素结合,可防止大肠癌向肝脏转移。京都大学遗传药理学教授武藤诚率领的研
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Molecular Characterization of Inflammation-Induced JNK/c-Jun Signaling Pathway in Connection with Tu
Tumor cells recruit inflammatory cells to the tumor site and transform them into
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Single-Domain VH Antibody Fragments from a Phage Display Library
The interaction between antigens and antibodies can be viewed as a protein engin
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Galectin-3 Binding and Metastasis
Galectin-3 is a member of a family of carbohydrate-binding proteins. It is prese
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细胞因子和重组蛋白溶解必读(一)
我们知道,PeproTech的所有细胞因子和蛋白均为冻干粉,这使得运输非常便捷,只要常温即可。而且,细胞因子和蛋白冻干粉非常稳定,在-20℃或-80℃条件下可保
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转染细胞不表达怎么办
问:最近做细胞转染,预筛选稳定克隆,载体为pEGFP-N1,pIERS现在是平行做几个基因,转然后,只有空载体和一个基因可以看到绿色荧光,其余均没有,这会是什么
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萤光素酶载体PGL3转染细胞要不要加G418
问:最近要做萤光素酶载体PGL3转染细胞,PGL3载体上面带有neo的抗性标记,有一个老师和我说要培养基里面要加G418筛选,但我查了一些PGL3载体转染的文献