Liposome Preparation

Liposome Preparation OBJECTIVE: Method for incorporating proteins into liposomes for liposome swelling assay or as an alternative antigen presentation method. REAGENTS: Dioleoyl Phosphatidyl Choline Buffer of choice / distilled water METHODS:

Dry 0.5 mmole of dioleoyl phosphatidyl choline under nitrogen in a disposable glass tube. Evacuate in dessicator under vacuum for 30 minutes. Add buffer / dH20 to required volume and scrape the sides of the glass tube to dislodge the lipid. Add protein at 1 mg/ul of lipid used. Vortex for 30 seconds. Sonicate twice in a bath sonicator at 7 degree for 15 sec.

This makes multilamellar vesicles that become small unilamellar vesicles (SUV) with prolonged sonication time. To make large unilamellar vesicles, use the extruder.