多克隆抗体

Making Antibody

・         Production of Polyclonal Antibody in Rabbit (Walter Steffen)Provides detailed protocol for immunizatioin, bleeding procedure, obtaining serum, precipitation of IgG.

・         GST-fusion protein injection into rabbits (IVAN J. DELGADO ORLIC)

・         How Do I make an Antibody against a Peptides (Pierce)

・         A fast and inexpensive method for raising high-specificity polyclonal antibodies in mice (Technical Tips Online)

・         Guide to Antipeptide Antibody (Perkin-Elmer)General Information about the design and synthesis of peptides for the production of anti-peptide antibodies, as well as an overview of the steps involved in the production of antibodies ­ immunization, collection of antisera, analysis of antisera, and antibody purification. Protocols for a number of these procedures are provided. 

o  Designing Antigens

o  Synthesizing Peptides

o  Conjugation of Peptides to Protein Carriers

o  Immunizations

o  Analysis of Antisera: Immunoassays

o  Purifying Antibodies 

• Preparation of Polyclonal Antibodies (PMCI)Immunization and antisera preparation  
• Making antibodies to synthetic peptides (Daniel T. O'Connor)  
• Preparation of Peptide-KLH Conjugates for Immunization (Mitshison Lab)  
• Testing Rabbit Bleeds by Elisa (Mitshison Lab)  
• Coupling Peptides to Resin for Affinity Purification (Mitshison Lab)  
• Purification of Anti Peptide Antibody (Mitshison Lab)

Others

・         Tips and hints for the storage of antibodies (Synaptic Systems)