Murine Monoclonal Antibody Development
Major advances in the analysis of biomolecular structure have emerged from the d
Major advances in the analysis of biomolecular structure have emerged from the development of highly specific monoclonal antibodies (MAb) by K�hler and Milstein in 1975 (1 ). MAb are produced from hybridoma cell lines that secrete a single species of antibody to a unique antigen. The advantages of MAb as reagents for antibody engineering are:
| 1. | They are extremely pure immunological reagents that can be produced by immunization with a heterogenous antigenic preparation; |
| 2. | They are homogenous with respect to affinity and specificity; |
| 3. | The specificity of the antibody is directed to a single epitope; |
| 4. | The desired MAb can be selected for activity, affinity, and subclass by applying appropriate screening methods; and |
| 5. | Virtually unlimited quantities of MAb can be produced. |
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