Chemokine Expression in Insect Cells

Recent progress in the collection of cDNA sequences by random sequencing reveals numerous genes of biological interest. Although a putative function may be predicted from deduced amino acid sequence, a rigorous proof of identity of a gene will require a demonstration of the appropriate biological activities of gene products. Thus, efficient expression of recombinant proteins of cloned genes is essential to obtain proteins sufficient for detailed biological and biochemical analysis. In this chapter, I will describe a rapid and efficient method to generate recombinant chemokines in insect cells (1 ,2 ).