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TOP10 chemically competent cells
OverviewThis protocol is a variant of the Hanahan protocol [1] using CCMB80 buff
2025-02-26 8 -
Extraction of Chromatin
Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College Exercise
2025-02-26 7 -
人白细胞介素5（IL-5）酶联免疫分析（ELISA）
人 白细胞介素5（IL-5） 酶联免疫 分析（ELISA）试剂 盒使用说明书本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样
2025-02-26 10 -
Chromatin Immunoprecipitation Assays for Myc and N-Myc
Myc and N-Myc have widespread impacts on the chromatin state within cells, both
2025-02-26 6 -
Visualization of Fluorescence-Tagged Proteins in Fission Yeast: The Analysis of Mitotic Spindle Dyna
Mitotic spindle microtubules pull chromosomes toward each pole to generate two d
2025-02-26 7 -
Fluorescence Recovery After Photobleaching
This chapter describes the use of microscope-based fluorescence recovery after p
2025-02-26 8 -
Differentiation of Human Embryonic Stem Cells into Mesenchymal Stem Cells by the Raclure Method
Mesenchymal stem cells also called mesenchymal stromal cells (MSCs) are multipot
2025-02-26 7 -
Simultaneous analysis of DNA content and surface immunophenotyp
Simultaneous analysis of DNA content and surface immunophenotype using gentle et
2025-02-26 11 -
UVB致皮肤角质形成细胞的损伤模型的制作方法
材料和方法 1 试剂:胎牛血清(北京军医兽医防治中心) ,DMEM培养液(GIBCO ,美国) ,碘化丙啶PI(Sigma) ,Triton2X100 (BDH
2025-02-26 7 -
兔成骨细胞与骨髓基质干细胞混合培养的实验研究
对骨髓基质干细胞进行改造,使其在保持快速生长的同时向成骨细胞方向分化是目前国内外研究的热点。实验发现,成熟的成骨细胞可以通过细胞间的直接接触作用促进骨髓基质干细
2025-02-26 7 -
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Ner
2025-02-26 6 -
Analysis of Signal Transduction: Formation of cAMP, cGMP, and Ins(1,4,5)P3 In Vivo and In Vitro
This chapter describes biochemical assays with which to analyze signal transduct
2025-02-26 7 -
Cytoplasmic mRNA Polyadenylation and Translation Assays
Vertebrate development is directed by maternally inherited messenger RNAs that a
2025-02-26 7 -
重链结合蛋白(heavy-chain binding protein, Bip)
Bip是重链结合蛋白的简称,因为它能够同IgG抗体的重链结合而得名。Bip是一类分子伴侣,属于Hsp70家族,在内质网中有两个作用。第一，Bip同进入内质网的未
2025-02-26 8 -
Double-Label Immunoelectron Microscopy for Studying the Colocalization of Proteins in Cultured Cells
Multiple label immunoelectron microscopy localizes and detects multiple antigens
2025-02-26 9
