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Imaging of Intracellular Hydrogen Peroxide Production with Hyper Upon Stimulation of Hela Cells with
Reactive oxygen species (ROS) regulate both normal cell functions by activating
2025-02-25 7 -
Methods for the Purification of HQ-Tagged Proteins
The HQ (H = histidine, Q = glutamine) tag is a small fusion tag that can be isol
2025-02-25 7 -
Culturing HEK 293 Cells
ReagentsMedium: 500 ml Dulbecco’s Modified Eagle Medium (Gibco #41966-029) 55 ml
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细胞生物基本方法：复苏
复苏方法1． 从液氮罐中取出安剖；有时因安剖未封严，浸入了液氮，取出后因安剖内液氮迅速气化而发生爆炸，因此应带有防护眼睛和手套。2． 迅速放入盛有 36 ℃～
2025-02-25 9 -
Xenopus Oocyte Wound Healing as a Model System for Analysis of Microtubule-Actin Interactions
Microtubule-actin interactions are fundamental to many cellular processes such a
2025-02-25 7 -
High Resolution Fluorescent In Situ Hybridization in Drosophila
Tissue-specific gene expression is a major determinant in the elaboration of cel
2025-02-25 8 -
An Improved Method for Generating and Identifying Human Induced Pluripotent Stem Cells
This chapter describes the methods we use to derive and characterize human induc
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Cultures of Human Adipose Precursor Cells
Recently, there has been rapidly growing interest in cell culture models that al
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Primary Mouse Keratinocyte Culture
Mouse epidermal keratinocytes have traditionally been difficult to grow in vitro
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Gene Expression in Mouse Oocytes by RNA-Seq
Second-generation sequencers such as the Illumina GAIIx make possible the study
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Localization of mRNA in Vertebrate Axonal Compartments by In Situ Hybridization
The conclusive demonstration of RNA in vertebrate axons by in situ hybridization
2025-02-25 9 -
The In Vivo Evaluation of Active-Site TOR Inhibitors in Models of BCR-ABL+ Leukemia
Preclinical evaluation of candidate anticancer compounds requires appropriate an
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Analysis of Small Hsp Phosphorylation
Small heat-shock proteins (sHsps) are involved in diverse biological phenomena a
2025-02-25 10 -
Electrical Impedance Spectroscopy for Rapid and Noninvasive Analysis of Skin Electroporation
Transient disruption of skin’s barrier properties using high-voltage pulses invo
2025-02-25 8 -
Preparation of Segmented and Polarity Marked Microtubules
Segmented and polarity-marked microtubules are very useful for many different ty
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