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Isolation and Functional Analysis of Mitochondria From the Nematode Caenorhabditis elegans
Mitochondria are essential organelles with central roles in diverse cellular pro
2025-02-25 10 -
ATP Production in Isolated Mitochondria of Procyclic Trypanosoma brucei
This chapter describes a luciferase-based protocol to measure adenosine triphosp
2025-02-25 11 -
Culture Systems: Fluid Dynamic Embryo Culture Systems (Microfluidics)
The tubal/uterine lumen is a dynamic environment in which oocytes, eggs, and ear
2025-02-25 10 -
Liposomes for Specific Depletion of Macrophages from Organs and Tissues
A liposome mediated macrophage “suicide” approach has been developed, based on t
2025-02-25 10 -
Isolation of a Sequence-Specific RNA Binding Protein, Polypyrimidine Tract Binding Protein, Using RN
Many important cellular processes are mediated by sequence-specific RNA binding
2025-02-25 18 -
Using Schizosaccharomyces pombe Meiosis to Analyze DNA Recombination Intermediates
The fission yeast Schizosaccharomyces pombe has many biological characteristics
2025-02-25 12 -
How to Assess and Study Cell Death in Dictyostelium discoideum
In this chapter, we describe how to conveniently demonstrate, assess, and study
2025-02-25 10 -
Protein-Fragment Complementation Assays for Large-Scale Analysis, Functional Dissection and Dynamic
Protein-fragment Complementation Assays (PCAs) are a family of assays for detect
2025-02-25 11 -
Ultrastructural Imaging of Cell Fusion in Caenorhabditis elegans
Caenorhabditis elegans is a well-established model system particularly suited fo
2025-02-25 12 -
Proteomics Method for Identification of Pseudopodium Phosphotyrosine Proteins
Cell migration requires actin/myosin-mediated membrane protrusion of a pseudopod
2025-02-25 13 -
MITOMYCIN C TREATMENT OF PMEFs
Cultures to be treated should be sub confluent ie actively growing.1. Add 1/20 v
2025-02-25 10 -
Quantitation of Orotic Acid in Urine Using Isotope Dilution-Selected Ion Gas Chromatography-Mass Spe
The measurement of urinary orotic acid excretion is an important test for establ
2025-02-25 12 -
Identification of Specific Protein-RNA Target Sites Using Libraries of Natural Sequences
RNA-binding proteins are involved in a variety of regulatory and developmental p
2025-02-25 9 -
Characterization of Human Adipose-Derived Stem Cells Using Flow Cytometry
One of the hallmark characteristics of human adipose-derived stem cells (hASCs)
2025-02-25 15 -
Microalgal Immobilization Methods
In this review, methods for the most common microalgal immobilization procedures
2025-02-25 12
