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Polo-Like Kinase-1: Activity Measurement and RNAi-Mediated Knockdown
Polo-like kinase-1 (Plk-1) is an important cell cycle regulatory kinase that has
2025-02-24 14 -
Gene Expression Analysis by In Situ Hybridization: Radioactive Probes
Over the last several decades, an understanding of the events involved in embryo
2025-02-24 12 -
Cryofixation Methods for Ultrastructural Studies of Dictyostelium discoideum
Cryopreservation methods, including rapid freezing, freeze-substitution, and low
2025-02-24 12 -
Retroposons: Genetic Footprints on the Evolutionary Paths of Life
Retroposons such as short interspersed elements (SINEs) and long interspersed el
2025-02-24 14 -
Isolation, Expansion, and Differentiation of Mouse Skin-Derived Precursors
The isolation and experimental manipulation of multipotent precursors is of incr
2025-02-24 11 -
Visualization of Complex Processes in Lipid Systems Using Computer Simulations and Molecular Graphic
Computer simulation has become an increasingly popular tool in the study of lipi
2025-02-24 13 -
The Use of RNA Probes for the Analysis of Gene Expression: Northern Blot Hybridization and Ribonucle
The isolation and characterization of RNA polymerases from the Salmonella phage
2025-02-24 16 -
Directed Differentiation of Dopamine Neurons from Human Pluripotent Stem Cells
Midbrain dopaminergic (mDA) neurons play a critical role in regulating postural
2025-02-24 13 -
Lineage Selection for Generation and Amplification of Neural Precursor Cells
Embryonic stem (ES) cells are derived from the epiblast of mouse blastocyst. The
2025-02-24 10 -
Enzymatic Dissociation and Culture of Normal Human Mammary Tissue to Detect Progenitor Activity
Normal human mammary tissue is composed of a glandular epithelium embedded withi
2025-02-24 13 -
Analysis of Skeletal Muscle Hypertrophy in Models of Increased Loading
Skeletal muscle is a highly adaptive tissue that modifies its size in response t
2025-02-24 14 -
Crystallization and Crystal Structure Determination of Ribonuclease A-Ribonuclease Inhibitor Protein
Ribonuclease inhibitor (RI) is a cytoplasmic protein of ∼50 kDa that tightly bin
2025-02-24 15 -
Lipidomic Analysis of Human Meibum Using HPLCMSn
High-pressure liquid chromatography–mass spectrometry (HPLC–MS) has become a de
2025-02-24 13 -
The Use of RAPD for Isolate Identification of Arbuscular Mycorrhizal Fungi
The basic polymerase chain reaction (PCR) ( 1 ), which consists of the exponenti
2025-02-24 11 -
Mechano-Transduction and Barrier Regulation in Lung Microvascular Endothelial Cells
Alterations in endothelial permeability are a hallmark of inflammation as well a
2025-02-24 9
