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A Detailed Protocol for Bacterial Artificial Chromosome Recombineering to Study Essential Genes in S
Bacterial artificial chromosome (BAC) recombineering is a novel technique for DN
2025-02-13 11 -
Monitoring DNA Damage During Cell Senescence
Cellular senescence is a state of irreversible cell cycle arrest, accompanied by
2025-02-13 10 -
Negative Stain Electron Microscopy of Microtubules
Negative Stain Electron Microscopy of Microtubules Negative staining is a rapid,
2025-02-13 10 -
Assaying Cell Cycle Checkpoints: Activity of the Protein Kinase Chk1
Eukaryotic cells regulate progression through the cell cycle in response to DNA
2025-02-12 10 -
Utilizing DNA Mutations to Trace Epithelial Cell Lineages in Human Tissues
Epithelial stem cells are typically multipotential and are likely the cell of or
2025-02-12 10 -
In Vitro Measurement of Phagocytosis and Killing of Cryptococcus neoformans by Macrophages
Macrophages are pivotal cells in immunity against a wide range of pathogens. The
2025-02-12 8 -
Novel and Immortalization-Based Protocols for the Generation of Neural CNS Stem Cell Lines for Gene
Transplantation of neural cells engineered to produce growth factors or molecule
2025-02-12 10 -
Production of Tissue-Engineered Skin and Oral Mucosa for Clinical and Experimental Use
Since the early 1990s, our understanding of how epithelial and stromal cells int
2025-02-12 7 -
细胞培养中几个错误认识
1 血清一定需要加热灭活 人们通常通过在56C加热30分钟的办法，来灭活血清中的补体，以及其中可能的微生物（比如支原体）的污染。加热灭活带来的问题是，血清中
2025-02-12 11 -
Monoclonal Antibodies Against Glycosphingolipids (GSLs)-Gangliosides
Free GSL molecules are poorly immunogenic using conventional immunization proced
2025-02-12 12 -
Primary Culture of Ovarian Cells for Research on Cell Interactions in the Hormonal Control of Steroi
Ovary is a polymorphic complex structure in which the cells are arranged in two
2025-02-12 9 -
Isolation and Establishment of Hair Follicle Dermal Papilla Cell Cultures
The isolation of hair follicle dermal papilla cells has become an important tech
2025-02-12 13 -
Assessing the Function of mTOR in Human Embryonic Stem Cells
We described a protocol for dissecting the function of an important serine/threo
2025-02-12 13 -
Vitrification of Plant Cell Suspensions
Deposition at cryogenic temperatures is generally considered to be the safest me
2025-02-12 14 -
Media Composition: Growth Factors
Despite the fact that the fundamental principle underlying the most common metho
2025-02-12 16
