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Cellular ELISA Protocol
Formalin Fixed Cell Plates1. Trypsinize confluent flasks2. Pool and count cells3
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CFU-A Assay for Measurement of the Antiproliferative Effects of Chemokines on Murine Early Hemopoiet
One of the more intriguing features of the hemopoietic system is the ability of
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Challenges and Opportunities in Fractionation of Recombinant Human Mannan-Binding Lectin
Mannan-binding lectin is an oligomeric protein of the innate immune system. Both
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Chaperonins in Phage Display of Antibody Fragments
The display of antibody fragments on the surface of filamentous bacteriophages (
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CHAPS Insoluble Floating Fraction Preparation
CHIFF preparationDevelop cells for at least 3 hrs to minimize protease activity.
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Characterisation of Viral Proteins that Inhibit Toll-Like Receptor Signal Transduction
Toll-like receptor (TLR) signalling involves five TIR adapter proteins, which co
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Characterization of HIV-1 Infection in the Humanized Rag2/c/ Mouse Model
Engraftment of immunodeficient mice with a human immune system (humanized mice)
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Characterization of Single-Domain Antibodies with an Engineered Disulfide Bond
Camelidae single-domain antibodies (VHHs) represent a unique class of emerging t
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Characterization of the Leishmanicidal Activity of Antimicrobial Peptides
This chapter describes the basic methodology to assay the activity of antimicrob
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Characterizing Immune Receptors from New Genome Sequences
Genome sequences are quickly being generated from a variety of organisms and pro
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Characterizing Somatic Hypermutation and Gene Conversion in the Chicken DT40 Cell System
The secondary immunoglobulin gene diversification processes, somatic hypermutati
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Chemical Synthesis of 2-O-Alkylated siRNAs
Chemical synthesis has been a major endeavor to create active siRNAs. The downre
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Chemistry and Biology of the ELISPOT Assay
Enzyme-linked immunospot, or ELISPOT, assay allows the detection of low frequenc
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Chemo-Enzymatic Production of O-Glycopeptides for the Detection of Serum Glycopeptide Antibodies
Protein microarray is a highly sensitive tool for antibody detection in serum. M
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Chemokine Expression in Insect Cells
Recent progress in the collection of cDNA sequences by random sequencing reveals
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