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Confocal Laser Scanning Microscopy Morphology and Apoptosis in Organogenesis-Stage Mouse Embryos
In our efforts to use confocal laser scanning microscopy for study of organogene
2025-02-12 9 -
ELISPOT (Enzyme-linked ImmunoSPOT)
IntroductionEnzyme-linked immunosorbent spot (ELISPOT) assays were originally de
2025-02-12 7 -
三羧酸循环(citric acid cycle)
由乙酰CoA和草酰乙酸缩合成有三个羧基的柠檬酸, 柠檬酸经一系列反应, 一再氧化脱羧, 经α酮戊二酸、 琥珀酸, 再降解成草酰乙酸。而参与这一循环的丙酮酸的三个
2025-02-12 10 -
细胞培养中的黑胶虫污染问题的处理
相关专题我们实验室最近一次的情况:我们前后从上海细胞中心买了三批细胞,细胞刚到的时候,观察均生长良好,密度适中,培养液清亮,也没有见小黑点,但是对细胞传代以后就
2025-02-12 12 -
The Cell Environment (including types of culture medium)
In general terms cultured cells require a sterile environment and a supply of nu
2025-02-12 7 -
杂交瘤细胞的制备方法
1) 瘤细胞培养(无特殊要求)骨髓瘤细胞呈悬浮或轻微附着形式生长,如附着性生长的细胞多时,用吸管轻轻吹打或轻轻叩击培养容器即可分离下来。通常要维持细胞于指数生长
2025-02-12 12 -
红细胞裂解的实验方法步骤
IntroductionPrior to using lymphoid tissue cell suspensions for flow cytometric
2025-02-12 9 -
Craniofacial Development of Avian and Rodent Embryos
In this overview, we examine approaches to the embryological development of the
2025-02-12 8 -
Analysis of Lymphocyte Development and Function Using the RAG-Deficient Blastocyst Complementation S
The RAG-deficient blastocyst complementation system (RBCS) represents a flexible
2025-02-12 7 -
去壁低渗法制备植物染色体标本实验
相关专题 一、实验目的: 1、掌屋植物染色体 标本的去壁低渗方法 2、了解中期染色体的形态结构 二、实验原理: 植物 细胞有很坚实的细胞壁,染色体很难像动物
2025-02-12 4 -
Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells
Cultured embryonic and adult skeletal muscle cells have a number of different us
2025-02-12 6 -
In Situ Detection of EpsteinBarr Virus and Phenotype Determination of EBV-Infected Cells
Epstein-Barr virus (EBV) establishes a lifelong infection of B cells. Consequent
2025-02-12 4 -
The Human Immunodeficiency Virus LTR-Promoter Region as a Reporter of Stress-Induced Gene Expression
Human immunodeficiency virus type-1 (HIV-1) replication and pro viral gene expre
2025-02-12 7 -
Single-Cell Enzymatic Dissociation of hESC Lines OxF1OxF4 and Culture in Feeder-Free Conditions
Experimental manipulation of hESCs has been hampered by their fragility and susc
2025-02-12 8 -
Cytotoxicity Assays Protocol
Cytotoxicity Assays ProtocolCell-mediated cytotoxicity was determined by using a
2025-02-12 4