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电穿孔与电融合技术
相关专题 【实验目的】 1．了解细胞电穿孔和电融合的基本原理 2．学习电穿孔和电融合基本技术 【实验原理】 当细胞置于非常高的电场中，细胞膜 就变得具有通透性
2025-02-12 5 -
293细胞的大规模培养的方法
相关专题 293细胞是腺病毒载体的包装细胞。腺病毒是继逆转录病毒后用于基因治疗研究的热门载体。直接将腺病毒载体导入人体内表达目的基因，治疗恶性肿瘤，心血管疾病
2025-02-12 9 -
Cellularity Measurements
Adipose tissue (AT) makes up 10–30% of body weight in normal-weight individuals,
2025-02-12 11 -
Chromatin Immunoprecipitation Coupled by Quantitative Real-Time PCR as a Tool for Analyzing Epigenet
Epigenetic regulation is one of the major players in gene expression control. Hi
2025-02-12 6 -
Transdermal Drug Delivery by Skin Electroporation in the Rat
Drug delivery across skin offers advantages over conventional modes of administr
2025-02-12 9 -
Retroviral-Mediated Transduction and Clonal Integration Analysis of Human Hematopoietic Stem and Pro
This chapter provides information on the methods used to introduce genes into hu
2025-02-12 5 -
Quantification of MicroRNAs, Splicing Isoforms, and Homologous mRNAs With the Invader Assay
The understanding of physiology and pathology requires accurate quantification o
2025-02-12 9 -
动物细胞大规模培养用生物反应器简介
相关专题 动物细胞培养 技术能否大规模工业化、商业化，关键在于能否设计出合适的生物反应器(bioreactor)。由于动物细胞与微生物细胞有很大差异，传统的微
2025-02-12 7 -
Functional Analysis of Nocturnin: A Circadian Clock-Regulated Gene Identified by Differential Displa
Within the retina there is a circadian clock that controls the 24-h timing of pr
2025-02-12 7 -
Immunohistochemistry of MMPs and TIMPs
Immunohistochemical techniques are a convenient method to identify the cells res
2025-02-12 7 -
Real-Time Analysis of Integrin-Dependent Transendothelial Migration and Integrin-Independent Interst
The role of integrins in leukocyte migration across endothelial barriers is wide
2025-02-12 5 -
Three-Dimensional Human Tissue Models of Wounded Skin
Human skin equivalents (HSEs) are in vitro tissues in which a fully differentiat
2025-02-12 6 -
Microscopic Computed Tomography-Based Virtual Histology of Embryos
Advances in imaging technologies and computational capabilities have made possib
2025-02-12 4 -
Intracellular Staining Protocol
1. Fix cells - Add16% formaldehyde directly into culture medium to obtain a fina
2025-02-12 4 -
Transgenic Mouse Models of Craniofacial Disorders
Disorders affecting craniofacial development represent a large fraction of birth
2025-02-12 6
