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Recording the Activity of ATP-Sensitive K+ Channels in Open-Cell Cell-Attached Configuration
The functional activity of ion channels is affected by their local environment,
2025-02-12 5 -
Testing Smell When It Is Really Vital: Behavioral Assays of Social Odors in the Neonatal Mouse
The initial interactions of mouse newborns with their mother are crucial for the
2025-02-12 6 -
Analysis of Endothelial Cell Migration Under Flow
The migration of endothelial cells (ECs) play an important role in embryonic vas
2025-02-12 5 -
Detection of c-Myc ProteinProtein Interactions and Phosphorylation Status by Immunoprecipitation
Co-immunoprecipitation is an invaluable technique in evaluating native protein–p
2025-02-12 7 -
Culture of Human Endothelial Cells from Umbilical Veins
The present protocol offers an economical option for the isolation and culture o
2025-02-12 8 -
Genetic Analysis of Cystic Fibrosis Airway Epithelial Cells
More than 500 different mutations have been described to date on the cystic fibr
2025-02-12 6 -
Sphingolipids in Macroautophagy
Sphingolipids are constituents of biological membranes. Ceramide and sphingosine
2025-02-12 7 -
A Genetic Model to Dissect the Role of Tsc-mTORC1 in Neuronal Cultures
Tuberous sclerosis complex (TSC) is an autosomal dominant disease caused by muta
2025-02-12 7 -
Radionuclide-Peptide Nucleic Acid in Diagnosis and Treatment of Pancreatic Cancer
Cancer of the exocrine pancreas will attack an estimated 31,000 Americans in 200
2025-02-12 8 -
用尼龙毛法分离B细胞
用尼龙毛法分离 B 细胞 1 )尼龙毛柱的制备 1. 取直接 3D ,长度约 10cm 的尼龙毛,用 0.2mol/L HCl 浸泡处理过夜。用双蒸水洗净 HC
2025-02-12 8 -
Biophysical Methods to Probe Claudin-Mediated Adhesion at the Cellular and Molecular Level
Claudins are a family of tetraspan membrane proteins that localize at tight junc
2025-02-12 10 -
Regulation of Transcription Factor Function by Targeted Protein Degradation: An Overview Focusing on
Regulation of protein degradation is an important mechanism by which concentrati
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Expression of Recombinant MMP-28 in Mammalian Cells
The expression of a recombinant MMP in a mammalian cell line can be useful, e.g.
2025-02-12 7 -
Bioinformatic Identification of Plant Peptides
Plant peptides play a number of important roles in defence, development and many
2025-02-12 9 -
Epidermal Cell Analysis by RT-PCR
Reverse transcription polymerase chain reaction is used for the semiquantitative
2025-02-12 9