-
Assessing Mitochondrial Potential, Calcium, and Redox State in Isolated Mammalian Cells Using Confoc
Mitochondria play a vital role in the regulation of intracellular calcium dynami
2025-02-12 7 -
Current Thoughts on the Therapeutic Potential of Stem Cell
Stem cells are considered as potential therapy for inflammatory disorders, tissu
2025-02-12 6 -
Murine Nonvolatile Pheromones: Isolation of Exocrine-Gland Secreting Peptide 1
Our search for a substance recognized by the vomeronasal neurons revealed that t
2025-02-12 6 -
Determination of Hydroxyurea in Serum or Plasma Using Gas Chromatography-Mass Spectrometry (GC-MS)
Hydroxyurea is an antineoplastic drug, which is also widely used in the treatmen
2025-02-12 7 -
Generation of Induced Pluripotent Stem Cells with CytoTune, a Non-Integrating Sendai Virus
One of the major obstacles in generating induced pluripotent stem cells for rese
2025-02-12 7 -
Studying Fertilization in Cell-Free Extracts: Focusing on Membrane/Lipid Raft Functions and Proteomi
Xenopus oocytes, eggs, and embryos serve as an ideal model system to study sever
2025-02-12 7 -
Cryopreservation of Mammalian Embryos: Slow Cooling
The benefits of banking mammalian embryos for long-term storage have long been r
2025-02-12 6 -
Derivation, Maintenance, and Induction of the Differentiation In Vitro of Equine Embryonic Stem Cell
We describe here the isolation and maintenance of pluripotent embryonic stem (ES
2025-02-12 7 -
Localization of Zona Pellucida Receptors on Live Sperm by Fluorophore-Conjugated Solubilized Zona Pe
The zona pellucida is an extracellular glycoprotein coat that surrounds the oocy
2025-02-12 11 -
Minimization of Proteins by Random Fragmentation and Selection
Protein-protein interactions are involved in most biological processes and are a
2025-02-12 5 -
Cytological Analysis of Meiosis in Fixed Drosophila Ovaries
Methods are described to analyze two different parts of the Drosophila ovary, wh
2025-02-12 5 -
Isolation and Purification of Plant Nucleic Acids: Genomic and Chloroplast DNA
The use of molecular protocols has expanded into virtually all branches of plant
2025-02-12 4 -
Hoechst 33342/PI双染色法
1．悬浮生长的细胞在培养状态下加入Heochst 33342，终浓度为1μg/ml；帖壁生长的细胞用含有0.02％EDTA的0.25％胰蛋白酶消化成单细胞悬液，
2025-02-12 4 -
体内肝损伤模型（酒精）
一、材料 纯系SD雄性大鼠，体重180g～200 g，由浙江大学医学院实验动物中心提供。食用酒精选用北京酿酒总厂出品的56度红星二锅头。 二、方法 将大鼠随机分
2025-02-12 4 -
293T细胞的一点培养心得
相关专题 293细胞293T细胞差点被我养死，幸好我又将它起死回生，一点心得。细胞传代：当细胞在细胞培养 瓶内长满达到80%时就要传代，一传二，24小时后再传代
2025-02-12 5
