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Engineering of a 3D Nanostructured Scaffold Made of Functionalized Self-Assembling Peptides and Enca
Three-dimensional (3D) in vitro models of cell culture aim to fill the gap betwe
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Analysis of Suppressor of Cytokine Signalling (SOCS) Gene Expression by Real-Time Quantitative PCR
The Suppressor of Cytokine Signalling (SOCS) proteins are a family of negative r
2025-02-27 21 -
细胞培养常见问题解析
相关专题1、如何选用培养基?培养某一类型细胞没有固定的培养条件。在MEM中培养的细胞，很可能在DMEM或M199中同样很容易生长。总之，首选MEM做粘附细胞培养
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Freezing cells in liquid nitrogen
Take off Media Trypsinate with 1ml x2 Dulbecco A trypsin Add 7ml Media Pipette u
2025-02-27 15 -
Analysis of Pyroptosis in Bacterial Infection
Eukaryotic cells undergo death by several different mechanisms: apoptosis, a cel
2025-02-27 14 -
DNA导入哺乳动物细胞常见的方法
名称 机制 转染效率 用途 优缺点 影响因素 磷酸钙转染法 内吞作用 20%细胞被转染 瞬时表达 1、简单有效 2、适用于贴壁、非贴壁细胞 3、常作首选方法
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Chlamydomonas reinhardtii: The Model of Choice to Study Mitochondria From Unicellular Photosynthetic
Chlamydomonas reinhardtii is a model organism to study photosynthesis, cellular
2025-02-27 12 -
Control of Vascular Tube Morphogenesis and Maturation in 3D Extracellular Matrices by Endothelial Ce
An important advance using in vitro EC tube morphogenesis and maturation models
2025-02-27 15 -
内吞（endocytosis）
大分子及颗粒物质进入细胞的方式。细胞膜对大分子物质是不通透的，细胞通过内吞摄入这类物质，首先，摄入物附着于细胞表面，被周围质膜逐渐包围，随之质膜内陷，最后分离下
2025-02-27 29 -
传代培养细胞染色体显示法
相关专题 1．培养细胞：取处于指数生长期、用较大瓶皿培养的、80％～90％汇合单层培养细胞。 2．加秋水仙素：使用最终浓度为0.02～0.8微克/毫升营养液，
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信号分子(signaling molecules)
信号分子是指生物体内的某些化学分子, 既非营养物, 又非能源物质和结构物质,而且也不是酶,它们主要是用来在细胞间和细胞内传递信息, 如激素、神经递质、生长因子等
2025-02-27 19 -
Monitoring Neuropeptides In Vivo via Microdialysis and Mass Spectrometry
Neuropeptides are important signaling molecules that regulate many essential phy
2025-02-27 14 -
RNA Interference Using a Plasmid Construct Expressing Short-Hairpin RNA
RNA interference (RNAi) is one of the most commonly used procedures for gene tar
2025-02-27 14 -
Micro-CGH: Microdissection-Based Comparative Genomic Hybridization
Comparative genomic hybridization (CGH) is a well-established technique in the f
2025-02-27 15 -
细胞培养中的无菌操作技术
1. 实验进行前，无菌室及无菌操作台(laminar flow) 以紫外灯照射30-60 分钟灭菌，以70% ethanol 擦拭无菌操作抬面，并开启无菌操作台
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