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Analysis of Signal Transduction: Formation of cAMP, cGMP, and Ins(1,4,5)P3 In Vivo and In Vitro
This chapter describes biochemical assays with which to analyze signal transduct
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Cytoplasmic mRNA Polyadenylation and Translation Assays
Vertebrate development is directed by maternally inherited messenger RNAs that a
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重链结合蛋白(heavy-chain binding protein, Bip)
Bip是重链结合蛋白的简称,因为它能够同IgG抗体的重链结合而得名。Bip是一类分子伴侣,属于Hsp70家族,在内质网中有两个作用。第一，Bip同进入内质网的未
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Double-Label Immunoelectron Microscopy for Studying the Colocalization of Proteins in Cultured Cells
Multiple label immunoelectron microscopy localizes and detects multiple antigens
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Immunohistochemical Staining of Cyclooxygenases with Monoclonal Antibodies
Immunohistochemistry is an important tool that is often used for the diagnosis o
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Preparation of cytospin from single cell suspension.
Cell number, speed and trivial details affect cytospin .Basic protocol:Prepare a
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细胞凋亡实验
相关专题 一、实验目的： 1、掌屋凋亡细胞的形态特征 2、学会用荧光探针 对细胞进行双标记来检测正常活细胞、凋亡细胞和坏死细胞的方法 二、实验原理 细胞死亡
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Noble Agar Assay
Description Cancer cells do not show anchorage and contact inhibition of growth.
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骨髓间充质干细胞的主要表面标志
1 骨髓间充质干细胞的发现和来源骨髓组织 中有多种细胞成分， 除基质细胞等已经分化的细胞外，还含有两类多潜能干细胞：造血干细胞和间充质干细胞。 1987 年F
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Staining Methods for cell
death Z. Xia 10/2/95 The simplest way: trypan blue. Dead cells stain blue Non-fi
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实验：用淋巴细胞分离液分离单个核细胞
相关专题 1）分离外周血中的单个核细胞（PBMC） 1．抽取正常人静脉血加到肝素抗凝管中，加等量含5～10IU/ml肝素的无血清缓冲液悬浮细胞。将细胞悬液小心
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细胞自发转化spontenous transformation
1.什么是细胞转化？何时会发生细胞转化？ 细胞转化是细胞发生遗传性状改变的一种变化，它的基础是DNA或基因的突变。从属性上说，基因突变导致生物体发生的转化，有利
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支原体(mycoplasma)
又称霉形体，是最简单的原核细胞，支原体的大小介于细菌与病毒之间,直径为0.1～0.3 um, 约为细菌的十分之一, 能够通过滤菌器。支原体形态多变,有圆形、丝状
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Generation of Induced Pluripotent Stem Cell Lines from Human Fibroblasts via Retroviral Gene Transfe
This chapter describes a protocol for deriving induced pluripotent stem cells (i
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Modulation of the Host Skeletal Muscle Niche for Donor Satellite Cell Grafting
Skeletal muscle tissue has a remarkable capability of regenerating in pathologic
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