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  • IP3受体(IP3 receptor)

    IP3受体(IP3 receptor)

    IP3受体是一种内质网通道蛋白, 由四个相对分子质量为260kDa的糖蛋白组成的四聚体。四个亚基组成一个跨膜的通道, 每个亚基都有IP3结合的部位, 当3~4个

    2025-02-11 13
  • High Resolution PCR Quantitation by AmpliSensor Assay

    High Resolution PCR Quantitation by AmpliSensor Assay

    A real-time kinetic tool for polymerase chain reaction (PCR) quantitation, Ampli

    2025-02-11 6
  • Analysis of Mitochondrial DNA by Two-Dimensional Agarose Gel Electrophoresis

    Analysis of Mitochondrial DNA by Two-Dimensional Agarose Gel Electrophoresis

    In higher vertebrates, the DNA of mitochondria takes the form of circular molecu

    2025-02-11 7
  • In Situ Zymography

    In Situ Zymography

    The techniques of Western blotting and immunocytochemistry are suitable for the

    2025-02-11 6
  • An In Vitro System to Evaluate the Scaffold Function of the RalA Effector Protein RalBP1

    An In Vitro System to Evaluate the Scaffold Function of the RalA Effector Protein RalBP1

    The in vitro kinase assay is an invaluable method to identify and characterize k

    2025-02-11 8
  • Reversion of Mouse Postimplantation Epiblast Stem Cells to a Nave Pluripotent State by Modulation of

    Reversion of Mouse Postimplantation Epiblast Stem Cells to a Nave Pluripotent State by Modulation of

    Mouse postimplantation epiblast cultured in activin and basic fibroblast growth

    2025-02-11 7
  • Using Fluorogenic Peptide Substrates to Assay Matrix Metalloproteinases

    Using Fluorogenic Peptide Substrates to Assay Matrix Metalloproteinases

    Catabolism of extracellular matrix (ECM) components has been ascribed to a famil

    2025-02-11 4
  • Detection and Characterization of Leishmania Parasites by DNA-Based Methods: Southern Blotting and P

    Detection and Characterization of Leishmania Parasites by DNA-Based Methods: Southern Blotting and P

    Leishmaniasis is a parasitic disease that is difficult to diagnose. This IS owin

    2025-02-11 8
  • Limiting Dilution Analysis of Murine Epidermal Stem Cells Using an In Vivo Regeneration Assay

    Limiting Dilution Analysis of Murine Epidermal Stem Cells Using an In Vivo Regeneration Assay

    Epidermal stem cells are of major importance for tissue homeostasis, wound repai

    2025-02-11 4
  • 氧还电位(oxidation-reduction potentials, redox potentials)

    氧还电位(oxidation-reduction potentials, redox potentials)

    由于不同的还原剂具有不同的电子传递电位,而氧化与还原又是偶联的,如NAD 和NADH.它们的差别主要是电子数量不同,所以二者间就有一个电位差, 即氧还电位。构成

    2025-02-11 5
  • Assessing Bad Sub-cellular Localization Under Conditions Associated with Prevention or Promotion of

    Assessing Bad Sub-cellular Localization Under Conditions Associated with Prevention or Promotion of

    Cells belonging to the monocyte/macrophage lineage are in general highly resista

    2025-02-11 6
  • Oocytes Microinjection Assay to Study the MAP-Kinase Cascade

    Oocytes Microinjection Assay to Study the MAP-Kinase Cascade

    The oocytes of several organisms—most frequently those of the African clawed toa

    2025-02-11 5
  • 细胞复苏之我见-经验总结

    细胞复苏之我见-经验总结

    在细胞复苏过程中,有多次复苏失败,最终在查阅了相关技术积累了足够的复苏技能后复苏成功,现将细胞复苏的操作程序和注意事项总结如下,望能为同行提供些参考。 【材料】

    2025-02-11 4
  • 几种细胞培养基M199中维生素含量的测定方法

    几种细胞培养基M199中维生素含量的测定方法

    相关专题 细胞培养基的配制 细胞培养专题细胞培养 基作为细胞工程重要的原材料,其品质的优劣直接影响到生物制品的质量,是生物制品企业非常重视的基础原料。而细胞培养

    2025-02-11 6
  • 植物多倍体诱发实验

    植物多倍体诱发实验

    相关专题 一 实验目的   1.了解人工诱导多倍体的原理,初步掌握用秋水仙素诱发多倍体的方法。   2.了解植物多倍体细胞染色体 加倍的特点 二 实验原理 植

    2025-02-11 10