-
Cloning of Variable Domains from Mouse Hybridoma by PCR
Some hybridoma cell lines are very low producers, difficult to cultivate, or ant
2025-07-14 6 -
Cloning Single-Chain Antibody Fragments (ScFv) from Hyrbidoma Cells
Despite the rising impact of the generation of antibodies by phage display and o
2025-07-14 4 -
Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format
This protocol describes the generation of monoclonal antibodies in single-chain
2025-07-14 4 -
Cochleates for Induction of Mucosal and Systemic Immune Responses
The vast majority of infectious diseases are caused by pathogens that infect muc
2025-07-14 4 -
Collagen-Induced Arthritis
Collagen-induced arthritis (CIA) is a polyarthritis induced by sensitization of
2025-07-14 4 -
Collecting Resident or Thioglycollate-Elicited Peritoneal Macrophages
Peritoneal macrophages are invaluable for gaining an understanding of innate imm
2025-07-14 4 -
Colloidal Gold Staining and Immunodetection in 2D Protein Mapping
This chapter extends the use of the technique described in Chapter 21 to two-dim
2025-07-14 4 -
Colloidal Gold Staining and Immunodetection in 2-D Protein Mapping
This chapter extends the use of the technique described in the previous chapter
2025-07-14 5 -
Colloidal Gold Staining and Immunoprobing on the Same Western Blot
Proteins, blotted from polyacrylamide gels onto nitrocellulose sheets (Western b
2025-07-14 5 -
Colloidal Gold/Streptavidin Methods
Biotin-avidin detection systems are widely used in both immunocytochemistry and
2025-07-14 4 -
Combining ELISPOT and ELISA to Measure Amounts of Cytokines Secreted by a Single Cell
Enzyme-linked immunospot (ELISPOT) assay allows for the determination of the fre
2025-07-14 4 -
Commercial Considerations for Immunoproteomics
The underlying drivers of scientific processes have been rapidly evolving, but t
2025-07-14 4 -
Comparative Properties of Polyclonal and Monoclonal Antibodies
This chapter was written with the express goal of introducing several basic conc
2025-07-14 4 -
Comparing Bacterial Membrane Interactions of Antimicrobial Peptides and Their Mimics
Interactions with bacterial membranes are integral to the mechanisms of action o
2025-07-14 5 -
Comparison of Immunotoxins Bearing a Single Saporin Molecule with Multiple Toxin Conjugates
Immunotoxins (IT) constructed by conventional chemical means using heterobifunct
2025-07-14 4
