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Electroporation Strategies for Genetic Manipulation and Cell Labeling
Electroporation has emerged as an effective method for cell labeling and manipul
2025-02-24 14 -
Enrichment and Clonal Culture of Hepatic Stem/Progenitor Cells During Mouse Liver Development
Liver regenerates after hepatectomy or chemical-induced injury. In contrast to c
2025-02-24 9 -
Primary Marrow-Derived Stromal Cells: Isolation and Manipulation
Marrow stromal cells (MSCs) are relatively rare cells difficult to visualize in
2025-02-24 15 -
Transplantation of Human Embryonic Stem Cells to the Chick Embryo
The traditional methods of studying the differentiation of human embryonic stem
2025-02-24 15 -
Use of Lipidomics for Analyzing Glycerolipid and Cholesteryl Ester Oxidation by Gas Chromatography,
Various analytical techniques have been adopted for the isolation and identifica
2025-02-24 12 -
缝隙连接(gap junction)
缝隙连接分布广泛,几乎存在于所有的动物细胞中。用超薄切片术可显示相邻两细胞的连接处的细胞质膜明暗相间七层结构,细胞间的缝隙约2纳米,其内有间隔的均匀排列的颗粒
2025-02-24 13 -
Microinjection into Xenopus Oocytes and Embryos
The ease of obtaining large numbers of Xenopus laevis eggs and oocytes together
2025-02-24 9 -
M-Current Recording from Acute DRG Slices
Electrophysiological recordings from an acutely sliced preparation provide infor
2025-02-24 11 -
前质体(proplastid)
前质体是质体的前身, 仅由内膜和外膜构成,膜内是少量的基质和DNA,但是没有LHC蛋白、叶绿素和电子传递系统。植物中的前质体随着在发育过程中所处的位置以及接受光
2025-02-24 9 -
The Problems and Pitfalls of Radioligand Binding
Radioligand binding is an extremely versatile technique that can be applied to a
2025-02-24 10 -
Differentiation of B Lymphocytes From Hematopoietic Stem Cells
Differentiation of B lymphocytes can be efficiently obtained when multipotent he
2025-02-24 10 -
贴壁细胞培养过程中支原体污染的几种救治方案
3.2 加温对支原体污染的治疗效果:经观察发现,加温8h后,荧光小点开始变少。16h细胞周围支原体几乎消失。进入24h开始细胞周围的荧光小点完全消失,但细胞开始
2025-02-24 11 -
High-Throughput Analyses of Gene Functions on a Cell Chip by Electroporation
Genome-wide functional annotation of genes is one of the major challenges in cur
2025-02-24 10 -
Disrupting Protein Complexes Using Tat-Tagged Peptide Mimics
Protein–protein interaction is a widely existing phenomenon and is essential for
2025-02-24 10 -
细胞培养目的与用途
1 科学研究:药物研究开发与基础研究 药物研究与开发 (1) 新药筛选:如化学合成药物药效研究,中药有效成分筛选与鉴定等. (2) 疫苗研究与开发:如病毒性疫苗
2025-02-22 11