-
A first step in defining the function of a novel gene is to determine its interactions with other ge
The difference between peptides and proteins (the subject of Chapter 2 ) is that
2025-02-07 8 -
Detection and Visualization of Protein Interactions with Protein Fragment Complementation Assays
A first step in defining the function of a novel gene is to determine its intera
2025-02-07 9 -
Human Embryonic Stem (ES) Cell Protocols――Splitting Human ES cells on MEFs
based on splitting onto one plateWarm collagenase IV split media to 37 C in a wa
2025-02-07 10 -
Vascular Progenitor Cell Mobilization
Blood vessel formation plays a key role in both physiologic and pathologic tissu
2025-02-07 9 -
Cytochemical Probes of Cycling and Quiescent Cells Applicable to Flow Cytometry
For convenience the term quiescent cells will be used in this chapter to define
2025-02-07 8 -
Poly(Ethylene Glycol) Crosslinked to Albumin as a Support for Enzyme Immobilization
Medical applications of enzymes are found in various areas, such as diagnosis, p
2025-02-07 11 -
Quantitative Polymerase Chain Reaction
Quantitative PCR (qPCR) has entered widespread use with the increasing availabil
2025-02-07 10 -
Chromosome Spreading and Immunofluorescence Methods in Saccharomyes cerevisiae
Visualization of meiotic chromosomes in the model organism S. cerevisiae has bec
2025-02-07 7 -
Fluorescence-Activated Cell Sorting Analysis of Mitochondrial Content, Membrane Potential, and Matri
Fluorescence-activated cell sorting (FACS) permits specific biologic parameters
2025-02-07 8 -
Glycosylation Profiling of Heterologous Proteins
The methylotrophic yeast Pichia pastoris has been widely exploited for its high-
2025-02-07 8 -
Mouse Epidermal Keratinocyte Culture
Cultured mouse epidermal keratinocytes provide a powerful model for studying epi
2025-02-07 5 -
In Vitro Spermatogenesis Using an Organ Culture Technique
Research on in vitro spermatogenesis has a long history and remained to be an un
2025-02-07 4 -
Serum Thawing Heat Inactivation
Serum Thawing & Heat Inactivation(Chris Cohick from JRH Biosciences catalogue)Ho
2025-02-07 5 -
Bacterial Phagosome Acidification Within IFN--Activated Macrophages: Role of Host p47 Immunity-Relat
Interferon-gamma IFN-γ)–induced remodeling of the bacterial phagosome for pathog
2025-02-07 8 -
-Tubulin and Microtubule-Binding Assays
Dynamic instability is a hallmark of the microtubule cytoskeleton. In order to r
2025-02-07 5
