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Isolation of Porcine Pancreatic Islets for Xenotransplantation
This chapter deals with a technique for isolating intact islets of Langerhans fr
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Production and Purification of Polyclonal Antibodies
Polyclonal antibodies are derived from multiple B-cell clones that have differen
2025-02-22 13 -
High-Resolution Molecular Localization by Freeze-Fracture Replica Labeling
The freeze-fracture technique splits the frozen lipid bilayer membrane into two
2025-02-22 11 -
An Optimized RNA Extraction Method from Archival Formalin-Fixed Paraffin-Embedded Tissue
Formalin-fixed and paraffin-embedded (FFPE) tissue is one of the most valuable r
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Assessment of Cardiac Conduction: Basic Principles of Optical Mapping
Extracellular recordings acquired from electrodes placed on the surface of cardi
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Derivation, Maintenance, and Characterization of Rat Embryonic Stem Cells In Vitro
The in vitro differentiation of mouse embryonic stem (ES) cells into different s
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G-蛋白偶联受体(G-protein linked receptor)
配体与受体结合后激活相邻的G-蛋白, 被激活的G-蛋白又可激活或抑制一种产生特异第二信使的酶或离子通道,引起膜电位的变化。由于这种受体参与的信号转导作用要与GT
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Transfection of Adipocytes and Preparation of Nuclear Extracts
The uniqueness of the adipose cell type must be considered before choosing a pro
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Utility of Array CGH in Molecular Diagnosis of Mitochondrial Disorders
Array comparative genomic hybridization (aCGH) is a powerful clinical diagnostic
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Quantitative Recovery of Biotinylated Proteins from Streptavidin-Based Affinity Chromatography Resin
The strong interaction between streptavidin and biotin is one of the most common
2025-02-22 13 -
Simple Experimental and Spontaneous Metastasis Assays in Mice
Many steps of the metastatic cascade can be reproduced in simple in vitro assays
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Functional Genomics by Gene-Trapping in Embryonic Stem Cells
The pace of sequencing whole genomes by far exceeds our increase of knowledge on
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原生质体(potoplast)
脱去细胞壁的细胞叫原生质体, 是一生物工程学的概念。如植物细胞和细菌(或其它有细胞壁的细胞)通过酶解使细胞壁溶解而得到的具有质膜的原生质球状体。动物细胞就相当于
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Development of ATP-Competitive mTOR Inhibitors
The mammalian Target of Rapamycin (mTOR)-mediated signaling transduction pathway
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Detecting Expression Patterns of Wnt Pathway Components in Sea Urchin Embryos
The animal–vegetal (A–V) axis is a maternally established asymmetry that is pres
2025-02-22 9
