-
Subtraction Hybridization for the Isolation of Strain-Specific Rhizobium DNA Probes
The principle of subtraction hybridization protocols is the removal from one cel
2025-02-22 12 -
The use of Saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins
Biochemical assay of proteomic libraries derived from the Saccharomyces cerevisi
2025-02-22 9 -
Measuring Redox Changes In Vivo in Leaves: Prospects and Technical Challenges
In leaves, the functioning of many key proteins under conditions promoting oxida
2025-02-22 10 -
Quantitation of Benzodiazepines in Blood and Urine Using Gas Chromatography-Mass Spectrometry (GC-MS
The benzodiazepine assay utilizes gas chromatography-mass spectrometry (GC-MS) f
2025-02-22 13 -
A FRET-Based Approach for Studying Conformational Changes of a Cytoskeleton-Related Tumor Suppressor
Changes in conformation are an important regulatory mechanism for a wide variety
2025-02-22 9 -
FISH Analysis of Human Pluripotent Stem Cells
Human pluripotent stem cells (PSCs) hold promise for treating a multitude of dis
2025-02-22 10 -
Immobilized Metal Ion Affinity Chromatography of Native Proteins
Immobilized metal affinity chromatography (IMAC) is a common place technique in
2025-02-22 11 -
Characterization of SMAD Phosphorylation and SMAD/Receptor Interaction
Signaling by transforming growth factor-(β superfamily members is initiated when
2025-02-22 10 -
Measurements of the Intracellular Stability of CPPs
Nowadays, the analysis of the uptake and intracellular distribution of cell-pene
2025-02-22 9 -
Isolation of Skin Dendritic Cells from Mouse and Man
Dendritic cells (DC) are crucial for the induction of immune responses and popul
2025-02-22 12 -
Application of Fluorescent Measurements for Characterization of RiboswitchLigand Interactions
Riboswitches are recently discovered messenger RNA motifs involved in gene regul
2025-02-22 10 -
MicroRNA Knock Down by Cholesterol-Conjugated Antisense Oligos in Mouse Organ Culture
Here, we detail a protocol to design and introduce sequence-specific cholesterol
2025-02-22 9 -
Colorimetric Assays for Screening Laccases
In this chapter, we describe colorimetric methods for screening laccases using n
2025-02-22 12 -
Site-Specific Integration in Human ESC Using Jump-In TI Technology
Engineering of human embryonic stem cells (hESC) offers a great potential tool f
2025-02-22 12 -
Preparation and Testing of Synthetic mRNA for Microinjection
The misexpression and overexpression of proteins by injection of synthetic mRNA
2025-02-22 10
