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Rubidium Efflux as a Tool for the Pharmacological Characterisation of Compounds with BK Channel Open
This chapter describes a method of assaying rubidium (Rb+ ) efflux as a measure
2025-02-06 6 -
ECM Degradation Assays for Analyzing Local Cell Invasion
Proteolytic degradation of extracellular matrix (ECM) is a critical step during
2025-02-06 13 -
Analyzing Import Intermediates of Mitochondrial Proteins by Blue Native Gel Electrophoresis
Blue native gel electrophoresis (BNGE) is a powerful tool for analyzing native p
2025-02-06 15 -
Managing Apheresis Complications During the Hematopoietic Stem Cell Collection
Adverse reactions may occur during the Hematopoietic Progenitor Cells via Aphere
2025-02-06 9 -
Isolation of Adult Mouse Stem Keratinocytes Using Magnetic Cell Sorting (MACS)
The functional and molecular characterization of mouse epidermal stem cells (SC)
2025-02-06 8 -
A Historical Perspective of the EGF Receptor and Related Systems
Since the isolation of epidermal growth factor (EGF) from mouse submaxillary gla
2025-02-06 8 -
Cysteinyl-Tagging of Integral Membrane Proteins for Proteomic Analysis Using Liquid Chromatography-T
Membrane proteomic analysis is of considerable interest due to the role of recep
2025-02-06 6 -
核纤层(lamina)
在与核质相邻的核膜内表面有一层厚30～160nm 网络状蛋白质, 叫核纤层, 对核被膜起支撑作用。核纤层由3种分子量为6～7万道尔顿的多肽亚单位α、β、γ所组成
2025-02-06 7 -
磷脂交换蛋白(phospholipid exchang proteins, PEP)
PEP是一种水溶性的载体蛋白,可以在不同的膜结合细胞器之间转移磷脂。转移的过程是: PEP首先与磷脂分子结合, 形成水溶性的复合物进入细胞质基质中, 通过自由扩
2025-02-06 8 -
Quantitative X-ray Imaging of Rodent Bone by Faxitron
This chapter describes the use of digital micro-radiography with the Faxitron ma
2025-02-06 5 -
Mechanical Properties of Bone Ex Vivo
The primary functions of bone are to do with support and protection – mechanical
2025-02-06 5 -
Tryptic Digestion of In-Gel Proteins for Mass Spectrometry Analysis
Identification and characterization of proteins are ultimately the goal in prote
2025-02-06 5 -
Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriation
Synchronized populations of large numbers of cells can be obtained by centrifuga
2025-02-06 5 -
Freezing and Thawing Cultured Cells
Freezing Cells: 1. Trypsinize cells and harvest in the normal way. 2. Count a 20
2025-02-06 5 -
The Generation and the Manipulation of Human Multipotent Adipose-Derived Stem Cells
In this chapter, we describe a method to isolate and to expand multipotent adipo
2025-02-06 5
