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Loss-of-Function Studies in Mouse Embryonic Stem Cells Using the pHYPER shRNA Plasmid Vector
RNA interference is widely used for loss-of-function studies in mammalian cells.
2025-02-21 17 -
Cationic α-Helical Peptides for Gene Delivery into Cells
Development of nonviral gene transfer techniques has progressed, particularly th
2025-02-21 17 -
Lipoplex Formation Using Liposomes Prepared by Ethanol Injection
Cationic liposomes composed of 3β-[N -(N� N� –dimethylaminoethane)carbamoyl] cho
2025-02-21 23 -
植物油凝素的制备
相关专题 植物 血凝素也称为植物凝集素(PHA),可自制也可购自商品。自制的方法常用生理盐水提取法。 (A)干品制备法(1)选广东鸡子豆10g,用蒸馏水冲
2025-02-21 18 -
Metalloproteases and the Degradome
Metalloproteases comprise a heterogeneous group of proteolytic enzymes whose mai
2025-02-21 16 -
Protocol for evaluating vascular permeability in wildtype
1) Female Tiel-PR Tg mice and wildtype control litter-mates aged 6-9 wks were ov
2025-02-21 14 -
动物体内转染试剂
相关专题 总有一种转染方法适合你摘要:由于RNAi实验技术的推广和应用,人们在体外培养细胞中进行RNAi实验已经比较容易。随着研究的深入,人们需要将研究扩展至更
2025-02-21 22 -
特征性研究:内源性GPCRs 细胞功能图研究
前言G-偶联蛋白受体(GPCRs),即 7-跨膜蛋白,是当今临床与科研药物的单一治疗靶点,也是迄今发现最大的靶点家族。据推测人基因组中,该家族的功能成员超过30
2025-02-21 18 -
Selection of Novel Eukaryotic DNA Polymerases by Mutagenesis and Genetic Complementation of Yeast
DNA-directed DNA polymerases have been broadly classified into seven families ba
2025-02-21 18 -
Preparing 48-Well Plate Feeders
Preparing 48-Well Plate FeedersIn preparing 48-well plates, a dilution must be m
2025-02-21 17 -
LINE-1 Elements: Analysis by Fluorescence In-Situ Hybridization and Nucleotide Sequences
Long-interspersed nuclear element-1 (LINE-1) is a non-terminal repeat transposon
2025-02-21 9 -
cos-7的转染方法讨论
相关专题 总有一种转染方法适合你问:cos-7的转染1、细胞的培养:我们在培养细胞时发现该细胞长的很快,不到2天的时间就长满了;消化的时候老是成块的掉,如果消化
2025-02-21 15 -
Embryonic Stem Cells for Osteo-Degenerative Diseases
Current orthopedic practice to treat osteo-degenerative diseases, such as osteop
2025-02-21 10 -
Development of Hematopoietic Stem and Progenitor Cells from Mouse Embryonic Stem Cells, In Vitro, Su
Differentiation of pluripotent embryonic stem (ES) cells can recapitulate many a
2025-02-21 13 -
Derivation of Human Embryonic Stem Cells in Xeno-Free Conditions
Human embryonic stem cells (hESC) have the potential to treat a wide range of di
2025-02-21 12