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Angiogenesis Assays in the Chick CAM
The growth of new blood vessels from pre-existing vascular elements, or angiogen
2025-02-21 15 -
Pharmacogenetics: Defining the Genetic Basis of Drug Action and Inositol Trisphosphate Analysis
Medicinal drugs do not always have clearly understood mechanisms of action, espe
2025-02-21 12 -
Analysis of RNA Structure and RNA-Protein Interactions in Mammalian Cells by Use of Terminal Transfe
Terminal transferase-dependent polymerase chain reaction (TDPCR) can be used aft
2025-02-21 12 -
Culture of Murine Megakaryocytes and Platelets from Fetal Liver and Bone Marrow
Megakaryocytes (MKs) are the largest hematopoietic cells in bone marrow. Since t
2025-02-21 12 -
Human Myometrial Smooth Muscle Cells and Cervical Fibroblasts in Culture: A Comparative Study
Uterine contractility and cervical tonicity change throughout the menstrual cycl
2025-02-21 17 -
Analysis of Tubulin Transport in Nerve Processes
In neurons, the molecular machinery for axonal growth and navigation is localize
2025-02-21 13 -
RNAi in the Hedgehog Signaling Pathway: pFRiPE, a Vector for Temporally and Spatially Controlled RNA
RNA interference (RNAi) has become an irreplaceable tool for reverse genetics in
2025-02-21 15 -
Purification and Identification of Protein Complexes That Control the Cell Cycle
The human Rad9-Hus1-Rad1 (9-1-1) and Rad17-RFC40-RFC38-RFC37-RFC36 (hRad17-RFC)
2025-02-21 10 -
In Ovo Gene Electroporation into Early Chicken Embryos
In chicken embryos, viral vectors have been successfully used to transfer foreig
2025-02-21 14 -
Generation of Human Embryonic Stem Cells Carrying Lineage Specific Reporters
The distinctive properties of human embryonic stem cells (hESCs) enable them to
2025-02-21 9 -
Identification, Characterization, and Differentiation of Human Prostate Cells
The prostate is organized as a classical exocrine gland and is composed of a com
2025-02-21 14 -
Biochemical Methods to Determine Cell-Surface Topography: Part A. Labeling of Oxidized Glycoproteins
This method selectively labels glycoproteins on the cell surface. Tritiated boro
2025-02-21 9 -
Chromatin Immunoprecipitation for Identifying Transcription Factor Targets in Keratinocytes
Protein–DNA interactions, such as those that are necessary for transcription, ar
2025-02-21 11 -
Generation of Mouse Embryos with Small Hairpin RNA-Mediated Knockdown of Gene Expression
We are using knockdown of gene expression in mouse embryos by constitutive expre
2025-02-21 12 -
Potential Zoonotic Infection of Porcine Endogenous Retrovirus in Xenotransplantation
Porcine endogenous retrovirus (PERV) is considered the major biosafety issue in
2025-02-21 16