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Frizzled-7-dependent Tissue Separation in the Xenopus Gastrula
Formation of tissue boundaries can be studied in a simple, inexpensive system, t
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Construction of Linker-Scanning Mutations Using PCR
In Chapter 25 we described linker scanning mutagenesis by oligonucleotide ligati
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Localization and MHC Class II Presentation of Antigens Targeted for Macroautophagy
Intracellular antigens can be presented on major histocompatibility complex (MHC
2025-02-20 10 -
Navigation to the Graveyard-Induction of Various Pathways of Necrosis and Their Classification by Fl
Apoptosis and necrosis reflect the program of cell death employed by a dying cel
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Isotope Labeling Strategies for Analysis of an Ion Channel Cytoplasmic Domain by NMR Spectroscopy
As large, multimeric, integral membrane proteins, ion channels pose technical ch
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Using PCR for Rapid Site-Specific Mutagenesis in Large Plasmids
Using polymerase chain reaction (PCR) it is possible to amplify a segment of DNA
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Dissecting Nucleases into Their Structural and Functional Domains: Mapping the RNA-Binding Surface o
The enzyme RNase III catalyzes the hydrolysis of the phosphodiester bond between
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TEM Visualization of Microtubules
LEVEL IIMaterials Coated grid for TEM0.1 M ammonium acetate5% ethanol saturated
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Zymography and Reverse Zymography for Detecting MMPs, andTIMPs
Zymography and reverse zymography are techniques used to analyze the activities
2025-02-20 14 -
Growth of Human Embryonic Stem Cells Using Derivates of Human Fibroblasts
The majority of human embryonic stem cell (hESC) lines have been derived and gro
2025-02-20 15 -
Studying Vascular Progenitor Cells in a Neonatal Mouse Model
While many murine models have been developed to study adult disease, animal rese
2025-02-20 14 -
Gene Expression Analysis Using Quantitative Reverse Transcription-Polymerase Chain Reaction and a Mu
Quantitation of very small amounts of specific messenger ribonucleic acids (mRNA
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Synthesis of Polyampholyte Comb-Type Copolymers Consisting of a Poly(L-lysine) Backbone and Hyaluron
Polycations have been used as nonviral gene carriers because the polycations and
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Isolation of Rat Intestinal Lymph DC
Dendritic cells (DCs) migrate constitutively from the intestine via the lymph to
2025-02-20 14 -
Dorsal Skinfold Chamber Preparation in Mice: Studying Angiogenesis by Intravital Microscopy
Since 1924, when the first transparent chamber model in animals was introduced b
2025-02-20 12
