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原代细胞分离培养与鉴定有哪些优点?
1.保持细胞的原始特性:原代细胞分离培养可以从组织或器官中分离出原始的细胞,这些细胞保留了体内组织的特性和相互作用。相比之下,细胞系(细胞株)经过长时间的传代培
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In Vitro Culture of Fetal Ovaries: A Model to Study Factors Regulating Early Follicular Development
Follicular development commences with the formation of primordial follicles, whi
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Claudins in a Primary Cultured Puffer Fish (Tetraodon nigroviridis) Gill Epithelium
A primary cultured gill epithelium from the model organism Tetraodon nigroviridi
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SAMe and HuR in Liver Physiology: Usefulness of Stem Cells in Hepatic Differentiation Research
S -Adenosylmethionine, abbreviated as SAM, SAMe or AdoMet, is the principal meth
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Microarray Technology for Mutation Analysis of Low-Template DNA Samples
Microarrays containing oligonucleotide mutation probes are emerging as useful pl
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B-Cell Signal Transduction: Tyrosine Phosphorylation, Kinase Activity, and Calcium Mobilization
Signal transduction by the B-cell antigen receptor (BCR) regulates development,
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Neural Differentiation of Human ES and iPS Cells in Three-Dimensional Collagen and Martigel Gels
In this chapter, we describe an effective and reproducible protocol for neural d
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High-Throughput Screening of Microtubule-Interacting Drugs
Drugs that affect microtubule dynamics are among the most effective anticancer a
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Screen for Oxidases by Detection of Hydrogen Peroxide with Horseradish Peroxidase
Oxidases remove hydrogen from substrates, transferring it to molecular oxygen to
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流式细胞仪在睡眠性阵发性血红蛋白尿的诊断中的应用
一 . 睡眠性阵发性血红蛋白尿 PNH睡眠性阵发性血红蛋白尿( PNH )是一种获得性造血干细胞异常,临床主要表现为骨髓丧失造血功能、血栓、慢性溶血性贫血急性发
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Counting Chromosomes in Intact Eggs
Chromosomal spreads are an established method to assess ploidy in different cell
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Viral Transformation of Epithelial Cells
Approximately 18% of human cancers have a viral etiology and the majority of the
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The MAP Kinase Signaling Cascades: A System of Hundreds of Components Regulates a Diverse Array of P
Sequential activation of kinases within the mitogen-activated protein (MAP) kina
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Genome-Wide Epigenetic Analysis of Human Pluripotent Stem Cells by ChIP and ChIP-Seq
Chromatin immunoprecipitation (ChIP) is used to evaluate the interaction of prot
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Determination of Proteasomal Activities
The proteasome is an important component of the intracellular system for the tur
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