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Feeder-Layer Free Culture System for Human Embryonic Stem Cells
Human embryonic stem cells (hESCs) are pluripotent stem cells derived from the i
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A Simple Method for Site-Specific Mutagenesis that Leaves the Rest of the Template Unaltered
Site-directed mutagenesis is an important component of modern molecular biology.
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Media Composition: Salts and Osmolality
The main components of embryo culture media are salts, which dissociate into the
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Bioreactor Cultivation of Functional Bone Grafts
The clinical demand for functional tissue-engineered bone grafts to regenerate b
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Genotyping of Mycobacterium tuberculosis Clinical Isolates Using IS6110-Based Restriction Fragment L
A number of phylogenetic studies of Mycobacterium tuberculosis have suggested a
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Trophoblast Differentiation: An In Vitro Model for Trophoblast Giant Cell Development
Trophoblast cells are situated between maternal and embryonic compartments and e
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Direct Sequencing of PCR Products with DNA-Binding Proteins
The polymerase chain reaction (PCR) has become widely established as a powerful
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Visualization of mRNA Expression in the Zebrafish Embryo
Examination of spatial and temporal gene expression pattern is a key step toward
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Towards a Standardized Protocol for the Generation of Monocyte-Derived Dendritic Cell Vaccines
For more than one decade patients have been treated with dendritic cell (DC) imm
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Fourier Transform Infrared Imaging of Bone
Fourier transform infrared imaging (FTIRI) is a technique that can be used to an
2025-02-20 10 -
Transient Overexpression of Genes in Neurons Using Nucleofection
Nucleofection is a transfection method used to introduce substrates such as cDNA
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Isolation and Culture of Mouse Satellite Cells
Muscle tissue culture provides a system for studying the growth and differentiat
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Differential mRNA Display: Adaption for In Vivo Studies of Diseased Tissues
This section focuses on the application of differential display in gaining insig
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Production of Knockdown Rats by Lentiviral Transduction of Embryos with Short Hairpin RNA Transgenes
The primary method for determining the function of a gene in rodents has been to
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Quantification of Cyclobutane Pyrimidine Dimers in Human Epidermal Stem Cells
The common procedures that are used to quantify cyclobutane pyrimidine dimers (C
2025-02-20 12
