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Visualization of Induced RNA in Single Bacterial Cells
Visualization of RNA in live cells is a challenging task due to the transient ch
2025-02-19 19 -
Isolation and Short-Term Culture of Human Epidermal Langerhans Cells
The human epidermis consists of a heterogeneous population of cells including ke
2025-02-19 20 -
Visualization of Spindle Behavior Using Confocal Microscopy
Numerous comparisons have shown that confocal imaging of fluorescently labeled s
2025-02-19 15 -
Yeast Protoplast Fusion
A major limitation to the results that could be achieved using traditional metho
2025-02-19 15 -
Immobilization of Enzymes and Proteins on Red Blood Cells
Immobilization of proteins on intact red blood cells (RBCs) can be valuable for
2025-02-19 16 -
The Measurement of Protein Degradation in Response to Oxidative Stress
Molecular oxygen is an excellent acceptor of electrons and is therefore employed
2025-02-19 14 -
Cell Viability Assays: Introduction
The measurement of cell viability plays a fundamental role in all forms of cell
2025-02-19 13 -
In Situ Hybridization with Nonradioactive Probes
In situ hybridization takes advantage of the reaction between two complementary
2025-02-19 16 -
Analysis of Fungal Gene Expression by Real Time Quantitative PCR
The Real-Time quantitative PCR (qPCR) method has become central for the quantifi
2025-02-19 12 -
Detection of Protein Glutathionylation
Recent studies indicate that protein glutathionylation is an important regulator
2025-02-19 17 -
导肽(leading peptide)
又称转运肽(transit peptide)或导向序列(targeting sequence),它是游离核糖体上合成的蛋白质的N-端信号。 导肽是新生蛋白N-端
2025-02-19 28 -
心肌细胞培养方法详述
配液:Hanks液、低糖DMEM液、高糖DMEM液、胰酶消化液、双抗液、碳酸氢钠液、盐酸液。 取鼠:鼠盆用棉铺好,取鼠。 准备:事先检查显微镜、磁力搅拌器及离心
2025-02-19 11 -
Identification of Genes Involved in Tumor-Like Properties of Embryonic Stem Cells
Embryonic stem (ES) cells are pluripotent stem cells derived from preimplantatio
2025-02-19 11 -
Analysis of Chromatin Composition of Repetitive Sequences: The ChIP-Chop Assay
Chromatin immunoprecipitation (ChIP) is a powerful method that allows to probe s
2025-02-19 15 -
Review of Molecular Mechanisms Involved in the Activation of the Nrf2-ARE Signaling Pathway by Chemo
Human exposures to environmental toxicants have been associated with etiology of
2025-02-19 14