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Restriction Enzymes
Restriction enzymes are endonucleases that recognize specific double-stranded DN
2025-02-19 18 -
Use of Fluorescence Spectroscopy to Study the Regulation of Small G Proteins
The Ras-like low-molecular-weight guanosine triphosphate (GTP)-binding proteins
2025-02-19 12 -
Techniques for the Study of Apoptosis in Bone
There has been great interest in the identifying the mechanisms by which apoptos
2025-02-19 14 -
Measurement of Low-Frequency DNA Breaks Using Nucleoid Flow Cytometry
The organization of eukaryotic DNA is increasingly well understood at each extre
2025-02-19 9 -
Assessing the Involvement of Telomerase in Stem Cell Biology
Highly proliferative cells including stem cells and cancer cells express high le
2025-02-19 16 -
Transplantation and Culture Techniques for the Analysis of Urodele Limb Regeneration
Altering the spatial relationships of embryonic or postembryonic tissues is one
2025-02-19 8 -
Identification and Characterization of Small GTPase-Associated Kinases
Rho-family signal-transduction cascades, which appear to be conserved in all euk
2025-02-19 10 -
Establishing Cell-Based Reporter Systems for the Analysis of hTERT Expression
Telomeres are the protective structures at the end of eukaryotic chromosomes. Te
2025-02-19 9 -
Raf/MEK/MAPK Signaling Stimulates the Nuclear Translocation and Transactivating Activity of FOXM1
The proliferation-associated transcription factor FOXM1 is essential for cell cy
2025-02-19 10 -
Immobilization of Enzymes on Inorganic Supports by Covalent Methods
Covalent binding of an enzyme to a support is probably the most interesting meth
2025-02-19 8 -
Differentiating Embryonic Stem Cells into Embryoid Bodies
Since the early 1980s, embryonic stem (ES) cells have been isolated from the inn
2025-02-19 9 -
Techniques in Protein Methylation
Proteins can be methylated on the side-chain nitrogens of arginine and lysine re
2025-02-19 9 -
Xenopus tropicalis Oocytes: More Than Just a Beautiful Genome
For more than 30 yr, Xenopus laevis has been the animal of choice for studying t
2025-02-19 10 -
Padlock Probes and Rolling Circle Amplification for Detection of Repeats and Single-Copy Genes in th
Padlock probes and rolling circle amplification are techniques which can be used
2025-02-19 10 -
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
General StrategyWe typically work with tissue culture, primary mammalian cells,
2025-02-19 15
