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转胞吞作用(transcytosis)
转胞吞作用是一种特殊的内吞作用,受体和配体在内吞中并未作任何处理,只是经细胞内转运到相反的方向, 然后通过胞吐作用, 将内吞物释放到细胞外,这种内吞主要发生在极
2025-03-07 62 -
显微镜放射自显影样品的制备及观察
相关专题 显微放射自显影就是运用放射性标记化合物掺入生物样品,以及用核乳胶记录生物样品内放射性同位素释放核射线的技术。根据放射性标记化合物的性质和银粒的分布部
2025-03-07 57 -
Monitoring Protein Kinase A Activities Using Expressed Substrate in Live Cells
Protein kinase A (PKA) activity is regulated by intracellular cyclic adenosine m
2025-03-07 41 -
Confirmation of the Presence of Drugs of Abuse in Urine
Urine screenings for drugs of abuse are performed for many reasons, two of the m
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小分子RNA(small RNA)
存在于真核生物细胞核和细胞质中,它们的长度为100到300个碱基(酵母中最长的约1000个碱基)。多的每个细胞中可含有105 ~106 个这种RNA分子,少的则
2025-03-07 60 -
Thin-Layer Gel Filtration: Determination of Protein Molecular Weights
For many years Sephadex gel filtration of proteins has been a valuable tool for
2025-03-07 50 -
Oocyte Extracts for the Study of Meiotic M-M Transition
In meiotic cell cycles, meiosis I (MI) is followed by meiosis II (Mil) without a
2025-03-07 41 -
胚胎干细胞培养操作规程
维持ES细胞处于未分化状态:ES细胞培养用含有ESGRO(白血病抑制因子)的高糖培养基来阻止细胞的分化。为细胞提供包被有0.1%明胶的平板作为粘附细胞的基质。建
2025-03-07 48 -
Rockland Dr.Moncada 分享: 如何获得更漂亮的流式结果
1. 如何降低背景 流式细胞实验优化过程中的一个关键步骤是试剂(抗体)的滴定。为了达到最佳效果,实验者必须确定抗原结合达到饱和所需抗体的最低量。这将帮助实验者提
2025-03-07 54 -
Imaging Hematopoietic Stem Cells in the Marrow of Long Bones In Vivo
Hematopoietic stem and progenitor cells (HSPCs) are located in the bone marrow i
2025-03-07 46 -
In Situ Hybridization of Whole-Mount Embryos
Since the early analyses of gene expression in the Drosophila embryo (1 ), whole
2025-03-07 41 -
Autoradiography of 1-and 2-Adrenoceptors
Receptor autoradiography is derived from receptor binding techniques that were o
2025-03-07 44 -
胚胎干细胞培养
Media and Solution required for ES Cell Culture (Bowtell Lab)Routine Culturing o
2025-03-07 51 -
肌动蛋白(actin)
肌动蛋白是微丝的结构蛋白, 以两种形式存在, 即单体和多聚体。单体的肌动蛋白是由一条多肽链构成的球形分子, 又称球状肌动蛋白(globular actin, G
2025-03-07 42 -
A Mammalian Cell-Based Assay for Screening Inhibitors of RNA Cleavage
RNA cleavage is a catalytic reaction which defines many types of RNA processing
2025-03-07 43