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An In Situ Hybridization Technique to Detect Low-Abundance Slug mRNA in Adherent Cultured Cells
In this chapter, we describe a simple and relatively rapid technique for detecti
2025-02-19 13 -
Vertical Arrays: Microarrays of Complex Mixtures of Nucleic Acids
Vertical arrays are microarrays that have complex mixtures of nucleic acids as a
2025-02-19 14 -
Fusion Protocol for the Production of Mouse Hybridomas
Hybridoma technology makes it possible to produce almost unlimited amounts of mo
2025-02-19 10 -
Use of RNA Interference to Investigate Cytokine Signal Transduction in Pancreatic Beta Cells
Type 1 diabetes (T1D) is a chronic autoimmune disease characterized by immune in
2025-02-19 10 -
An Assay for Human Chemosignals
Like all mammals, humans use chemosignals. Nevertheless, only few such chemosign
2025-02-19 12 -
Pluripotency: Capacity for In Vitro Differentiation of Undifferentiated Embryonic Stem Cells
Embryonic stem (ES) cells, the pluripotent cells of early embryos have been succ
2025-02-19 10 -
Isolation of Protein Complexes Involved in Mitosis and Cytokinesis from Drosophila Cultured Cells
The identification of all the individual components that constitute the plethora
2025-02-19 14 -
质体(plastid)
质体是植物细胞中由双层膜包裹的一类细胞器的总称, 这类细胞器都是由共同的前体:前质体分化发育而来, 包括:叶绿体、白色体、淀粉质体、有色体、蛋白质体、油质体等。
2025-02-19 10 -
Cdc34 蛋白（Cdc34 protein）
Cdc34蛋白是E2遍在蛋白结合酶，与E3遍在蛋白连接酶(SCF)联合作用，降解期周期蛋白�Cdc28复合物的抑制物，从而激活S期周期蛋白激酶，起始DNA
2025-02-19 14 -
Analysis of Mammary Gland Morphogenesis
Mammary gland development is marked by numerous cellular changes that begin in t
2025-02-19 12 -
Immunohistochemical Analysis of mTOR Activity in Tissues
mTOR is a key regulator of cell growth and size, and its activity is often dysre
2025-02-19 11 -
The Animal Cap Assay
Over the last 10 years, the animal cap of the Xenopus laevis embryo has proved t
2025-02-19 9 -
Genetic Manipulation of Human Embryonic Stem Cells by Transfection
One of the great advantages of embryonic stem (ES) cells over other cell types i
2025-02-19 11 -
Micro-CT Technique for Three-Dimensional Visualization of Human Stem Cells
Micro-CT offers high spatial resolution of the distribution of stem cells and pr
2025-02-19 13 -
Detection of Gene Expression in Mouse Embryos and Tissue Sections
Analysis of gene expression patterns is central to the study of embryonic develo
2025-02-19 11
