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Isolation and Purification of Functionally Intact Mitochondria from Plant Cells
The isolation of mitochondria from plant cells that display similar biochemical
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S期促进因子(S phase-promoting factor,SPF)
与MPF相似，芽殖酵母的S期促进因子(S phase-promoting factor,SPF),也是异质二聚体, 一个是Cdc28, 另一个是在G1期起作用的
2025-02-18 12 -
PEG介导的动物细胞融合技术
相关专题 细胞融合(cell fusion)或细胞杂交(cell hybridization)是指真核细胞通过介导和培养，两个或多个细胞合并成一个双核或多核细
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细胞分泌(cell secretion)
动物细胞和植物细胞将在粗面内质网上合成而又非内质网组成部分的蛋白和脂通过小泡运输的方式经过高尔基体的进一步加工和分选运送到细胞内相应结构、细胞质膜以及细胞外的过
2025-02-18 10 -
Examination of the Brain Mitochondrial Lipidome Using Shotgun Lipidomics
Contamination from subcellular organelles and myelin has hindered attempts to ch
2025-02-18 12 -
Dorsal Axis Duplication as a Functional Readout for Wnt Activity
The easy accessibility, distinctive features of early cleavage stage embryos and
2025-02-18 12 -
High-Throughput FACS Method for Directed Evolution of Substrate Specificity
Directed evolution is a powerful technology for the isolation of enzyme variants
2025-02-18 11 -
Quantification of PARP Activity in Human Tissues: Ex Vivo Assays in Blood Cells and Immunohistochemi
Poly (ADP-ribosyl)ation of proteins is a posttranslational modification mediated
2025-02-18 14 -
Fate of Desmosomal Proteins in Apoptotic Epidermal Cells
In epidermal cells desmosomes represent major sites of basolateral cell-cell con
2025-02-18 10 -
ISH Probes Derived from BACs, Including Microwave Treatment for Better FISH Results
Besides the well-known applications of bacterial artificial chromosomes (BACs) i
2025-02-18 9 -
Detection of Ubiquitination in 2DE
Ubiquitination involves the tagging of proteins with one (mono-) or more (poly-)
2025-02-18 11 -
Redox-Sensitive Green Fluorescent Protein: Probes for Dynamic Intracellular Redox Responses. A Revie
The quantification of transient redox events within subcellular compartments, su
2025-02-18 12 -
Analysis of Tissue-Specific Gene Expression Using Laser Capture Microdissection
Epithelial tissues exhibit optimal conditions for studying cellular differentiat
2025-02-18 11 -
In Vivo SELEX in Vertebrate Cells
Iterative selection strategies have been widely used to enrich specific RNA mole
2025-02-18 15 -
Mammalian Lectin as Tool in Glycochemistry and Histochemistry With Relevance for Diagnostic Procedur
Carbohydrates represent a fundamental building unit of living organisms. Many co
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