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Electroporation-Mediated Gene Transfer into Hepatocytes
The study of gene expression regulation relies on the introduction of foreign DN
2025-02-18 21 -
Synthesis and Use of Spin-Labeled Lipids for Studies of the Transmembrane Movement of Phospholipids
The first measurement of the transmembrane diffusion of phospholipids in membran
2025-02-18 18 -
细胞生物基本方法：神经组织细胞培养
神经组织细胞培养1． 获取脑组织后，先仔细剥除脑膜和血管等纤维成分，置入 Hanks 液中漂洗 1 ～ 2 次后，置于 30 ～ 50 倍的 Hanks 液中，
2025-02-18 19 -
Antisense-Mediated Inhibition of Protein Synthesis: Rational Drug Design, Pharmacokinetics, Intracer
There is hardly any class of drugs for which the term “ratronal drug design” is
2025-02-18 15 -
Establishment and Maintenance of In Vitro Cultures of Human Retinal Pigment Epithelium
The retinal pigment epithelium (RPE) is a layer of multipotential cells of neura
2025-02-18 11 -
Protein Extraction for 2DE
Our protein extraction protocol for two-dimensional gel electrophoresis (2DE) wa
2025-02-18 12 -
Freezer and Cell Line Database
Very useful Microsoft Access database program (486kb) for managing your cell lin
2025-02-18 9 -
Assay of Phospholipase D Activity in Cell-Free Systems
Phospholipase D (PLD) enzymes are present in all animal and plant species and ha
2025-02-18 9 -
Murine C3H10T1/2 Multipotential Cells as an In Vitro Model of Mesenchymal Chondrogenesis
Bone, muscle, fat, and cartilage are differentiated adult tissues derived from e
2025-02-18 14 -
Flow Cytometry and Immunoselection of Human Stem Cells
Human hematopoietic stem cells (HSCs) can be obtained from a variety of hematopo
2025-02-18 12 -
Reprogramming of B Cells
Cellular reprogramming is an interplay between the original starting cell’s plas
2025-02-18 10 -
Harvesting Hematopoietic Cells from Mice
Materials 4 mice from each genotype 4 Ly5 mice Buckets with wet ice 3x Bucket wi
2025-02-18 17 -
Three-dimensional In vitro Angiogenesis in the Rat Aortic Ring Model
Angiogenesis is a complex sequential process involving endothelial activation, b
2025-02-18 18 -
Immunofluorescence Detection of the Cytoskeleton and Extracellular Matrix in Tissue and Cultured Cel
“A picture is worth a thousand words” goes the proverb. A poor picture however c
2025-02-18 18 -
Staining Methods for cell death
The simplest way: trypan blue. Dead cells stain blue Non-fixed cells: FDA(fluore
2025-02-18 22
