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培养基制备的基础实验
相关专题 细胞培养基的配制 细胞培养专题目的要求1.掌握一般培养基 制备的原则和要求。2.熟悉一般培养 基制备的过程。操作步骤一、培养基的制备原则和要求培养基是
2025-02-18 18 -
Generation of a Pool of Human Platelet Lysate and Efficient Use in Cell Culture
Human platelets represent a promising source of bioactive substances as growth f
2025-02-18 10 -
N-连接糖基化(N-linked glycosylation)
新合成蛋白进行糖基化修饰的一种方式。糖通过与蛋白质的天冬氨酸的自由NH2基连接,所以将这种糖基化称为N-连接的糖基化。这一过程在在内质网中进行的。糖基化的第一步
2025-02-18 12 -
单链断裂重组模型(model of single-strand breaks recombination)
该模型认为:同源染色体在联会期间,一对同源染色体各有一条单链DNA断裂,然后要发生重接,不过在重接的过程中发生了错误,不是原来断开的染色体重新连接起来而是交换连
2025-02-18 11 -
Gel Electrophoresis-Based Proteomics of Senescent Tissues
Cellular aging is a fundamental biological process, and mass spectrometry-based
2025-02-18 12 -
N-连接糖基化(N-linked glycosylation)
新合成蛋白进行糖基化修饰的一种方式。糖通过与蛋白质的天冬氨酸的自由NH2基连接,所以将这种糖基化称为N-连接的糖基化。这一过程在在内质网中进行的。糖基化的第一步
2025-02-18 19 -
单链断裂重组模型(model of single-strand breaks recombination)
该模型认为:同源染色体在联会期间,一对同源染色体各有一条单链DNA断裂,然后要发生重接,不过在重接的过程中发生了错误,不是原来断开的染色体重新连接起来而是交换连
2025-02-18 11 -
Gel Electrophoresis-Based Proteomics of Senescent Tissues
Cellular aging is a fundamental biological process, and mass spectrometry-based
2025-02-18 15 -
RAP-PCR Using RNA from Tissue Microdissection
Cell culture provides powerful models for studying cell biology, but many pathol
2025-02-18 18 -
Deoxyribonuclease I (EC 3.1.21.1) and II (EC 3.1.22.1)
From Chapter 1 on nucleases, we know that the term DNase refers to an enzyme tha
2025-02-18 20 -
Measurement of Activities of Cyclobutane-Pyrimidine-Dimer and (6-4)-Photoproduct Photolyases
The adverse biological consequences of unrepaired toxic UV-B-induced photoproduc
2025-02-18 14 -
体外细胞培养对细胞培养基几点要求
相关专题体外培养的细胞直接生活在培养基中,因此培养基应能满足细胞对营养成分、促生长因子、激素、渗透压、pH等诸多方面的要求。营养成分1. 氨基酸:所有细胞都需要
2025-02-18 11 -
EB病毒诱导淋巴细胞永生化实验方法
实验步骤: 1. EB病毒液的制备 复苏B95.8细胞株,使用培养基为10% FCS RPMI-1640 逐步添加培养基至细胞长至对数生长期
2025-02-18 11 -
Lipid Microdomains and K+ Channel Compartmentation: Detergent and Non-Detergent-Based Methods for th
The traditional view of the plasma membrane as a uniform cellular envelope forme
2025-02-18 17 -
Ultrasound-Responsive Liposomes
Ultrasound-responsive liposomes are drug-loaded liposomes that contain a small a
2025-02-18 13