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  • Small-Molecule Collection and High-Throughput Colorimetric Assay to Identify PARP1 Inhibitors

    Small-Molecule Collection and High-Throughput Colorimetric Assay to Identify PARP1 Inhibitors

    During the last few years, poly(ADP-ribose)polymerase (PARP) proteins became a v

    2025-01-21 4
  • 组成型分泌途径(constitutive secretory pathway)

    组成型分泌途径(constitutive secretory pathway)

    在这种分泌途径中, 运输小泡持续不断地从高尔基体运送到细胞质膜,并立即进行膜的融合,将分泌小泡中的蛋白质释放到细胞外, 此过程不需要任何信号的触发, 它存在于所

    2025-01-21 4
  • Detection of NF-B Activity in Skeletal Muscle Cells by Electrophoretic Mobility Shift Analysis

    Detection of NF-B Activity in Skeletal Muscle Cells by Electrophoretic Mobility Shift Analysis

    An electrophoretic mobility shift assay (EMSA) is a common and invaluable techni

    2025-01-21 6
  • Methods for Iodination of Active TGF, TGF Receptor Crosslinking and Immunoprecipitation of TGF-Recep

    Methods for Iodination of Active TGF, TGF Receptor Crosslinking and Immunoprecipitation of TGF-Recep

    Radioiodinated transforming growth factors ( TGF-βs) are critical reagents for f

    2025-01-21 4
  • Analysis of Neuromuscular Junctions: Histology and In Vivo Imaging

    Analysis of Neuromuscular Junctions: Histology and In Vivo Imaging

    The formation of new synapses within neuronal circuits is considered a primary m

    2025-01-21 10
  • Feeder-Independent Maintenance of Human Embryonic Stem Cells and Directed Differentiation into Endot

    Feeder-Independent Maintenance of Human Embryonic Stem Cells and Directed Differentiation into Endot

    Human embryonic stem cells (hESCs) represent a promising source of cells for mod

    2025-01-21 8
  • Covisualization of Methylcytosine, Global DNA, and Protein Biomarkers for In Situ 3D DNA Methylation

    Covisualization of Methylcytosine, Global DNA, and Protein Biomarkers for In Situ 3D DNA Methylation

    DNA methylation and histone modifications are key regulatory mechanisms in cellu

    2025-01-21 8
  • Methods for Analysis of Ligand-Induced RNA Conformational Changes

    Methods for Analysis of Ligand-Induced RNA Conformational Changes

    Encoded within many RNA sequences is the requisite information for folding of in

    2025-01-21 7
  • Sources and Derivation of Human Embryonic Stem Cells

    Sources and Derivation of Human Embryonic Stem Cells

    Human embryonic stem cells (hESCs) are pluripotent cells derived from the inner

    2025-01-21 7
  • Master Cell Bank

    Master Cell Bank

    Master Cell BankAuthor: Nanci DonackiSource: Contributed by Nanci DonackiAbstrac

    2025-01-21 5
  • PNA-In Situ Hybridization Method for Detection of HIV-1 DNA in Virus-Infected Cells and Subsequent D

    PNA-In Situ Hybridization Method for Detection of HIV-1 DNA in Virus-Infected Cells and Subsequent D

    We describe in situ hybridization protocols using peptide nucleic acid (PNA) as

    2025-01-21 4
  • Recording Ion Channels in Isolated, Split-Opened Tubules

    Recording Ion Channels in Isolated, Split-Opened Tubules

    Ion channels play key roles in physiology. They function as protein transducers

    2025-01-21 4
  • Raman Micro-Spectroscopy as a Non-invasive Cell Viability Test

    Raman Micro-Spectroscopy as a Non-invasive Cell Viability Test

    The number of techniques to identify, quantify and characterise cell death is ra

    2025-01-21 5
  • Tiny Molecular Beacons for in vivo mRNA Detection

    Tiny Molecular Beacons for in vivo mRNA Detection

    The molecular beacon technology is an established approach for visualizing nativ

    2025-01-21 8
  • Saponin Lysis of RBCs

    Saponin Lysis of RBCs

    CuratorsJingyang Chen, Seattle Biomedical Research Institute, 307 Westlake Ave N

    2025-01-21 4