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Culture of Human Embryonic Stem Cells (hESC)
All cell lines are initially grown according to the supplier's protocols but
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Purification and Depletion of RNP Particles by Antisense Affinity Chromatography
Antisense oligonucleotides made of 2′-O -alkyl RNA are useful reagents for the s
2025-02-18 11 -
Site-Directed Mutagenesis In Vitro by Megaprimer PCR
Among the various mutagenesis procedures based on polymerase chain reaction (PCR
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Models for Gain-of-Function and Loss-of-Function of MMPs: Transgenic and Gene Targeted Mice
The most powerful approach for studying gene function in an intact animal is to
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Prenatal Diagnostics on Uncultured Amniocytes
Fluorescence in situ hybridization (FISH) in prenatal diagnostics permits the de
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Methods for Creating Mutations in C. elegans That Extend Lifespan
The principle of commonly used methods to create mutations in the nematode Caeno
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Mouse Models of Viral Infection: Influenza Infection in the Lung
Respiratory viral infections are a major cause of morbidity and mortality. Prote
2025-02-18 11 -
Freezing and Thawing cells
Freezing and Thawing cellsFreezing It is best to freeze cells that are growing r
2025-02-18 10 -
Adult Cell Fate Reprogramming: Converting Liver to Pancreas
Regenerative medicine aims at producing new cells for repair or replacement of d
2025-02-18 15 -
Molecular Modeling and Molecular Dynamics Simulations of Membrane Transporter Proteins
In silico methods are increasingly being used to address problems of biological
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Oral and Pharyngeal Epithelial Keratinocyte Culture
Primary human oral epithelial cells are readily available and have been recently
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Analysis of Change
When the same subjects or laboratory animals are observed across a set of differ
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Immunoglobulin Class Switching: In Vitro Induction and Analysis
During an immune response, B lymphocytes can switch expression of immunoglobulin
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Gene Delivery to Mesenchymal Stem Cells
Successful gene therapy technology relies on the delivery of the therapeutic pro
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Preparation of Template DNA and Labeling Techniques
A variety of probes can be used for in situ hybridization, depending on the appl
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