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蛋白质寻靶(protein targeting)
游离核糖体合成的蛋白质在细胞内的定位是由前体蛋白本身具有的引导信号决定的。不同类型的引导信号可以引导蛋白质定位到特定的细胞器，如线粒体、叶绿体、细胞核和过氧化物
2025-01-18 9 -
High Pressure Freezing, Electron Microscopy, and Immuno-Electron Microscopy of Tetrahymena thermophi
Preservation of Tetrahymena thermophila basal body ultrastructure for visualizat
2025-01-18 8 -
染色体实验技术分析
染色体分裂指数低：患者处于非常时期（感染期、放、化疗期）： 培养基营养成份不良； 培养基PH偏低或偏高；
2025-01-18 6 -
Cocultivation of Human Oral Keratinocytes and Human Osteoblast-Like Cells
Head and neck reconstruction transplants often require a bony structure but also
2025-01-18 6 -
Cryopreservation of Shoot-Tips and Meristems
The ability to regenerate whole plants from cryopreserved, meristematic shoot ti
2025-01-18 6 -
Induction of DNA Double-Strand Breaks by Electroporation of Restriction Enzymes into Mammalian Cells
The introduction of restriction endonucleases into mammalian cells in culture pr
2025-01-18 11 -
Generation and Use of Epitope-Tagged Receptors
Epitope tagging of a receptor involves introducing a defined amino acid sequence
2025-01-18 5 -
Cystic Fibrosis Airway Epithelial Cell Culture
Cystic fibrosis (CF) is the most frequent (incidence around l/2500 live births)
2025-01-18 6 -
Characterization of B-Cell Maturation in the Peripheral Immune System
In the periphery different populations of B cells can be identified, correspondi
2025-01-18 9 -
Studying Ligand Efficacy at G Protein-Coupled Receptors Using FRET
Drug “ligands” that bind G protein-coupled receptors (GPCRs) can either stimulat
2025-01-18 7 -
Evaluating the Localization and DNA Binding Complexity of Histones in Mature Sperm
The paternal genome in many animal taxa is efficiently packaged into the sperm n
2025-01-18 5 -
Slice Cultures for Study of Microglia
Culture of isolated microglia from dissociated cortical tissue has promoted the
2025-01-18 5 -
Production of Transgenic Mice with Yeast Artificial Chromosomes
The stable introduction of recombinant DNA into the germline of mammals is one o
2025-01-18 5 -
Methods to Monitor Fatty Acid Transport Proceeding Through Vectorial Acylation
The process of fatty acid transport across the plasma membrane occurs by several
2025-01-18 10 -
Affinity Capture and Solid-Phase Sequencing of Biotinylated PCR Products
The time and labor required for generating nucleotide sequence information has b
2025-01-18 5
