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自体组装(self assembly)
生物大分子借助本身的力量自行装配成高级结构，现代的概念应理解为不需要模板和酶系的催化, 以别于模板组装和酶效应组装。其实，这种组装也需要一种称为分子伴侣的蛋白介
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核糖体
核糖体不是由生物膜构成的，它是由蛋白质和RNA 构成的复合体。由大小两个亚基组成。核糖体是蛋白质合成的场所。附着在内质网上的核糖体合成的蛋白质主要有两类：一类是
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Nucleotide Metabolism and Cell-Cell Interactions
Interactions between the vascular endothelium and polymorphonuclear leukocytes (
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FISH on Sperms, Spermatocytes and Oocytes
It is well known that chromosome in situ hybridization allows the unequivocal id
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细胞转染方法
物理方法 电穿孔(electroporition) 显微注射(microinjection) 化学方法 磷酸钙转染(calcium phosphate tran
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Tissue Culture Media
Tissue Culture MediaWe use two different kinds of media. Most cells are grown in
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减数分裂
有性生殖的生物在生殖细胞形成前的某个时期进行的一种特殊方式的有丝分裂，其二倍体原始生殖细胞内染色体复制一次之后，细胞连续分裂两次，使生殖细胞中染色体数目比原始
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QUALITATIVE ANALYSIS OF DNA FRAGMENTATION BY AGAROSE GEL ELECTROPHORESIS
1. IntroductionThe present protocol provides a method for DNA separation of frag
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Cell Surface Marker and Cell Cycle Analysis, Hedgehog Signaling, and Flow Cytometry
Detailed cytological analysis of cells undergoing differentiation often reveals
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Isolation of Murine Adipose-Derived Stem Cells
Murine models of obesity or reduced adiposity are a valuable resource for unders
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Analysis of Genomic Aberrations Using Comparative Genomic Hybridization of Metaphase Chromosomes
Comparative genomic hybridization (CGH) allows the global screening of copy numb
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Preparation of Isolated Periportal or Perivenous Hepatocytes from Rat Liver
There are a number of functional, metabolic differences between periportal and p
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Isolation and Growth of Adipose Tissue-Derived Stem Cells
Fat tissue provides a rich and easily accessible supply of stem cells that featu
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In Vitro Clonal Culture of Human Hematopoietic Progenitor Cells
Human hematopoietic tissue represents a complex developmental system that is clo
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Specific Cell Cycle Synchronization with Butyrate and Cell Cycle Analysis
Synchronized cells have been invaluable in many kinds of cell cycle and cell pro
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