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DIRECT ELISA PROTOCOL
Buffers and Reagents: Bicarbonate/carbonate coating buffer (100 mM) - antigen or
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Direct Immunofluorescence Labeling
Description This is the method for direct immunofluorescence labeling; that is,
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Direct Immunofluorescent Labeling of Cells
In their original immunofluorescent labeling technique, Coons and colleagues emp
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Directed Evolution of T-Cell Receptors for Binding Superantigens
A T-cell recognizes two major classes of antigens. Binding of the αβ T-cell rece
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Discovery and Customized Validation of Antibody Targets by Protein Arrays and Indirect ELISA
Because of our access to human genome data and ever improving genome sequencing
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Discovery of HostViral Protein Complexes During Infection
Viruses have co-evolved with their hosts, developing effective approaches for hi
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Discriminating Between the Paracellular and Transcellular Routes of Diapedesis
Leucocyte transendothelial migration (TEM) or diapedesis is pivotal in leucocyte
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Disorders of Neutrophil Function: An Overview
Primary disorders of neutrophil function result from impairment in neutrophil re
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Display of Antibody Chains on Filamentous Bacteriophage
The construction of very large combinatorial libraries whereby antigen-binding f
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Dissection of the Antiviral NK Cell Response by MCMV Mutants
Our understanding of virus control by natural killer (NK) cells relies mainly on
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Disulfide-Stabilized Fv Fragments
The design and generation of disulfide-stabilized Fv fragments (dsFv’s) addresse
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DNA As an Adjuvant
DNA is a complex macromolecule with immunological properties that depend on base
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DNA Hydrolysis by Antibodies
Catalysis of various chemical transformations by antibodies opens broad perspect
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DNA Vaccination: An Update
The volume entitled DNA Vaccines: Methods and Protocols was published in this se
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DNA Vaccine Design
The purpose of this chapter is to present basic strategies for the construction
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