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Expression from DNA Injected into Xenopus Embryos
Expression of genes by introduction of DNA into developing embryos can be used t
2025-01-16 12 -
Crystal Violet Assay
This is a simple assay useful for obtaining quantitative information about the r
2025-01-16 7 -
Double Immunohistochemistry with Horseradish Peroxidase and Alkaline Phosphatase Detection Systems
We describe here a protocol optimized for formalin-fixed paraffin-embedded tissu
2025-01-16 7 -
Isolation and Purification of Rabbit Osteoclasts
Newborn rabbits provide a useful and readily available source of authentic matur
2025-01-16 8 -
Comparative Phosphorylation Site Mapping From Gel-Derived Proteins Using a Multidimensional ES/MS-Ba
Understanding how phosphorylation regulates the behavior of individual proteins
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Fluorescence Methods for Evaluating Lipoplex-Mediated Gene Delivery
The biological activity of cationic liposome/DNA complexes (“lipoplexes”) is str
2025-01-16 7 -
Platelet Life Span and Apoptosis
Like many nucleated mammalian cells, the life and death of the anucleate platele
2025-01-16 4 -
Human Embryonic Stem (ES) Cell Protocols――Embryonic Bodies
Let human ES cells grow until the colonies are large and the cells are pretty pi
2025-01-16 4 -
Expression and Function of Claudins in Hepatocytes
Tight junctions of hepatocytes play crucial roles in the barrier to keep bile in
2025-01-16 9 -
High-Content Imaging and Analysis of Pluripotent Stem Cell-Derived Cardiomyocytes
Human pluripotent stem cells (hPSC) are investigated as a source of authentic hu
2025-01-16 12 -
巨噬细胞分型与免疫治疗
一、巨噬细胞分型巨噬细胞根据其激活状态和所处的微环境不同，可以分为多种亚型，首先是M1型和M2型。M1型巨噬细胞：主要由Th1细胞因子（如IFN-γ）或脂多糖（
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巨噬细胞分型意义
巨噬细胞：免疫系统的多面手巨噬细胞，作为一类广泛分布于全身各组织中的免疫细胞，它们不仅负责吞噬并消化病原体、细胞残骸及异物，还参与调节免疫反应、促进组织修复与再
2025-01-16 7 -
巨噬细胞分型的科研应用
一、疾病机制研究①癌症研究：巨噬细胞可分为M1型和M2型，它们在肿瘤微环境中表现出截然不同的功能。M1型巨噬细胞具有抗肿瘤作用，而M2型巨噬细胞则促进肿瘤生长和
2025-01-16 7 -
RNA免疫沉淀（RIP）实验原理
——RIP实验：基因表达调控的侦探工具想象一下，基因是生命的蓝图，而RNA则是这张蓝图上跳跃的音符，它们将遗传信息转化为蛋白质的功能指令。然而，并非所有RNA都
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RNA免疫沉淀（RIP）实验步骤流程详解
一、细胞裂解①准备细胞：将细胞培养至状态良好，吸除培养基。②清洗细胞：用10 mL冰冷的PBS洗涤培养瓶或平板上的细胞两次，以去除培养基和其他杂质。③收集细胞：
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