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Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures
Primary mouse embryonic fibroblasts (PMEFs) have a number of properties that mak
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Glomerular Epithelial and Mesangial Cell Culture and Characterization
The advent of in vitro culture techniques has enabled the culture of homogeneous
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Matrix Assembly, Cell Polarization, and Cell Survival: Analysis of Peri-Implantation Development Wit
A variety of mutations, including those affecting laminin expression and basemen
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Isolation and Purification Method of Mouse Fetal Hepatoblasts
During development, liver precursors constitute a valuable source of pluripotent
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Genome-Wide Analysis of RNA Extracted from Isolated Mitochondria
Isolating mitochondria by subcellular fractionation is a well-established method
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Assays of Lipolytic Enzymes
Free fatty acids (FAs) derived from adipose tissue (AT) are, quantitatively, the
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Preparing Mitochondria from Rat Liver
Liver is a convenient source for functional intact mitochondria for a number of
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Isolation and Culture of Bone-Forming Cells (Osteoblasts) from Human Bone
The most conspicuous function of the osteoblast is the formation of bone. During
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Tracking Nanoparticles in Three-Dimensional Tissue-Engineered Models Using Confocal Laser Scanning M
Here we describe a method for imaging the position of nanoparticles within a 3D
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分子细胞生物学(molecular biology of the cell)
以细胞为对象, 主要在分子水平上研究细胞生命活动的分子机制, 即研究细胞器、生物大分子与生命活动之间的变化发展过程, 研究它们之间的相互关系, 以及它们与环境之
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细胞死亡(cell death)
细胞死亡的一般定义是细胞生命现象不可逆的停止。细胞死亡有两种形式:一种为坏死性死亡(necrosis),是由外部的化学、物理或生物因素的侵袭而造成的细胞崩溃裂解
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ChIP on Chip Assays: Genome-Wide Analysis of Transcription Factor Binding and Histone Modifications
Deregulation of transcriptional activity of many genes has been causatively link
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兔白细胞介素6(IL-6)酶联免疫分析(ELISA)
兔白细胞介素6 (IL- 6 ) 酶联免疫 分析( ELISA )试剂 盒使用说明书本试剂仅供研究使用 目的:本试剂盒用于测定兔血清,血浆及相
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Processing mRNA 3' Ends In Vitro
The 3′ ends of most nonhistone mRNAs in mammalian cells are generated by the end
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Use of Confocal Microscopy for Three-Dimensional Imaging of Neurons in the Spinal Cord
Confocal microscopy provides a powerful and efficient tool for studying the morp
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