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Mapping the Lipolytic Proteome of Adipose Tissue Using Fluorescent Suicide Inhibitors
Lipases are responsible for the hydrolysis of acylglycerols and cholesteryl este
2025-02-14 11 -
Gene Targeting by Oligonucleotides in Keratinocytes
Oligonucleotide-directed gene alteration produces a targeted deoxyribonucleic ac
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Combined Pulldown and Time-Lapse Microscopy Studies for Determining the Role of Rap1 in the Crosstal
The coordinate modulation of the cellular functions of cadherins and integrins p
2025-02-14 12 -
Study of Skeletal Myogenesis in Cultures of Unsegmented Paraxial Mesoderm
Recent developments in the field of skeletal myogenesis have identified signalin
2025-02-14 14 -
Cell Cycle Synchronization for the Purpose of Somatic Cell Nuclear Transfer (SCNT)
Somatic cell nuclear transfer (SCNT) is a technically and biologically challengi
2025-02-14 8 -
Broad Spectrum Drug Screening Using Electron-Ionization Gas Chromatography-Mass Spectrometry (EI-GCM
A liquid–liquid extraction (LLE) of drugs and internal standard (promazine) is p
2025-02-14 10 -
Use of Escherichia coli for the Production and Purification of Membrane Proteins
Individual types of ion channels and other membrane proteins are typically expre
2025-02-14 10 -
Isolation of Mitochondria From Procyclic Trypanosoma brucei
The mitochondrion of the parasitic protozoon Trypanosoma brucei shows a number o
2025-02-14 10 -
Engineering Elements for Gene Silencing: The Artificial MicroRNAs Technology
Small RNA (sRNA)-mediated gene silencing constitutes a powerful tool for the mol
2025-02-14 14 -
The Stem Cell Laboratory: Design, Equipment, and Oversight
This chapter describes some of the major issues to be considered when setting up
2025-02-14 15 -
Direct Sequencing by Thermal Asymmetric PCR
Direct sequencing of PCR products (1 ) has proven to be a powerful method in the
2025-02-14 13 -
Fluorescent Glycerolipid Probes: Synthesis and Use for Examining Intracellular Lipid Trafficking
The synthesis, characterization, and use of fluorescently labeled lipid probes t
2025-02-14 14 -
Differential Gene Expression in ES-Derived Neural Stem Cells by Using RT-PCR
Embryonic stem (ES) cells hold promise to treat a variety of disease. The major
2025-02-14 11 -
Isolation of Sperm Nuclei and Nuclear Matrices from the Mouse, and Other Rodents
The isolation of mammalian sperm heads from their tails is complicated by the re
2025-02-14 15 -
Monitoring of the Dynamics of Epithelial Dome Formation Using a Novel Culture Chamber for Long-Term
Epithelial tissue guarantees proper performance of many organs, e.g., the kidney
2025-02-14 13
