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Methods to Measure Membrane Potential and Permeability Transition in the Mitochondria During Apoptos
Mitochondrial membrane permeabilization (MMP) constitutes an early event of the
2025-02-14 10 -
Experimental Identification of MicroRNA Targets by Immunoprecipitation of Argonaute Protein Complexe
MicroRNAs (miRNAs) represent a class of small noncoding RNAs that negatively reg
2025-02-14 9 -
Microscopic Techniques for Studying Sperm-Oocyte Interaction During Fertilization and Early Embryoni
The union between sperm and oocyte is species-specific, and the courses of event
2025-02-14 16 -
Immobilization of Enzymes and Cells: Some Practical Considerations
The technology for immobilization of cells and enzymes evolved steadily for the
2025-02-14 10 -
Structural Analysis of Protein-RNA Interactions With Mass Spectrometry
We present a high-resolution mass spectrometric footprinting approach enabling t
2025-02-14 12 -
关于动物血清的常见问题及处理方法
最近在培养细胞中使用胎牛血清,由于发现有沉淀,不知是否变质,在查阅相关文献也没有得到确切的答案.因此不敢再用原来的血清,导致浪费.在这提醒大家做细胞培养时,要注
2025-02-14 18 -
Chemical Assays for Proteins
The choice of a protein assay for membranes and membrane proteins is dependent o
2025-02-14 12 -
Generation of Human-Induced Pluripotent Stem Cells (hiPSCs) Using Episomal Vectors on Defined Essent
Human-induced pluripotent stem cells (iPSCs) are an important potential source o
2025-02-14 10 -
Isolation and Culture of Human Colon Epithelial Cells Using a Modified Explant Technique Employing a
Colorectal cancer, originating in tissues of epithelial origin, is one of the mo
2025-02-14 11 -
Introduction to Nucleocytoplasmic Transport: Molecules and Mechanisms
Nucleocytoplasmic transport, the exchange of matter between nucleus and cytoplas
2025-02-14 11 -
Analysis of Biomarkers of Caloric Restriction in Aging Cells
Caloric restriction (CR) has been extensively documented for its profound role i
2025-02-14 12 -
常用封闭剂选择的窍门
相关专题 生物实验中的封闭剂杂交膜上有很多非特异性的蛋白质结合位点,为防止这些位点与抗体结合引起非特异的染色和背景,一般用惰性蛋白质或非离子去污剂封闭膜上的未结
2025-02-14 10 -
Selective Extraction of Fragmented DNA from Apoptotic Cells for Analysis by Gel Electrophoresis and
A characteristic feature of apoptosis is activation of an endonuclease(s), which
2025-02-14 13 -
Identification of Mutated p53 in Cancers by Nongel-Sieving Capillary Electrophoretic SSCP Analysis
The tumor suppressor gene, p53, lies on chromosome 17p, and has been examined in
2025-02-14 11 -
Osteogenic Differentiation of Embryonic Stem Cells in 2D and 3D Culture
Osteoblasts are the cells that contribute to the formation and function of bone
2025-02-13 19