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Embryonic Stem Cells as a Model for the Physiological Analysis of the Cardiovascular System
Embryonic stem (ES) cells have the potential to proliferate infinitely in vitro
2025-01-16 6 -
核孔复合物(nuclear pore complexs,NPCs)
核被膜上有许多孔, 称为核孔( nuclear pore ),是细胞核膜上沟通核质与胞质的开口, 由内外两层膜的局部融合所形成, 核孔的直径为80~120nm。
2025-01-16 6 -
Prothrombinase Complex as a Tool to Assess Changes in Membrane Phospholipid Asymmetry
An important topic in membrane biochemistry is the determination of the sidednes
2025-01-16 5 -
Identification of Clock Genes Using Difference Gel Electrophoresis
Proteomics is the study of the complete set of proteins encoded by the genome. T
2025-01-16 6 -
Optimizing Stem Cell Grafting into the CNS
Grafting neural stem cell to facilitate repair after central nervous system (CNS
2025-01-16 7 -
Analyzing Double-Strand Repair Events in Drosophila
Targeted manipulation of the genome is used to analyze gene expression, genome s
2025-01-16 4 -
微管(microtubule)
微管是直径为24-26nm的中空圆柱体。外径平均为24nm, 内径为15nm。微管壁大约厚5nm,微管通常是直的, 但有时也呈弧形。细胞内微管呈网状和束状分布,
2025-01-16 16 -
Novel Designed Probes for the Characterization of Oxidative Stress in Biological Fluids, Cells, and
Oxidative stress (OS) is linked to the development of human diseases. Early iden
2025-01-16 11 -
High-Resolution Fluorescence In Situ Hybridization to Detect mRNAs in Neuronal Compartments In Vitro
The localization of specific mRNAs into dendrites and/or axons is an important m
2025-01-16 8 -
Restriction Endonucleases and Their Uses
Restriction endonucleases, which cleave DNA in a site-specific manner, are a fun
2025-01-16 9 -
琼脂糖凝胶电泳试剂
以下是琼脂糖凝胶电泳所需的主要试剂及其相关信息的清晰归纳:一、 琼脂糖①作用:作为凝胶基质,用于制作凝胶板。②浓度:琼脂糖的浓度会影响凝胶的孔径和分离效果
2025-01-15 11 -
琼脂糖凝胶电泳注意事项
㈠、样品制备①确保样品纯度:提高RNA或DNA样品的纯度,避免杂质干扰。使用高质量的提取试剂盒,并遵循严格的提取步骤。②调整样品浓度:根据实验需求调整样
2025-01-15 15 -
亚细胞定位实验是什么?
——以下是关于亚细胞定位实验的详细解析:一、实验定义亚细胞定位实验旨在揭示细胞内各种生物大分子的精确定位,这对于理解它们的生物学功能、相互作用以及细胞的整体运行
2025-01-15 10 -
亚细胞定位分析
一、什么是亚细胞定位?亚细胞定位,简而言之,就是确定某种蛋白或表达产物在细胞内的具体存在部位,如细胞核、细胞膜、线粒体、叶绿体等。这一过程对于理解基因的作用机
2025-01-15 20 -
详细描述GFP融合蛋白表达法的实验过程
1. 实验准备基因克隆:首先,需要获取目标蛋白的基因序列,并将其与GFP基因序列进行融合。这通常通过分子生物学技术(如PCR扩增、基因拼接等)完成,确保
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