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Stress Response Profiles in Human Fibroblasts Exposed to Heat Shock or Oxidative Stress
The upregulation of stress proteins is an important step in the cellular defense
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体外肝细胞损伤模型建立（CCl4和H2O2）
1 大鼠肝细胞的分离和培养 大鼠4％戊巴比妥麻醉，门静脉插管，先以无钙灌流液灌流，继以37℃通入O2的Ⅳ型胶原酶灌流液继续循环灌流15 min。将肝脏移至一平皿
2025-01-14 6 -
Lentiviral Vector-Mediated Gene Transfer in Embryonic Stem Cells
The major limitations in gene transduction to embryonic stem (ES) cells are (1)
2025-01-14 6 -
In Vitro Assays for Neural Stem Cell Differentiation
Neural stem cells (NSCs) are an undifferentiated population of cells residing in
2025-01-14 9 -
Rapid and Systematic Transfer and Recovery of Large BACs/PACs into Mammalian Cells by HAEC Retrofitt
Studies of the human genome have prompted the development of several cloning sys
2025-01-14 9 -
A Transplant Model for Human Epidermal Skin Regeneration
This protocol describes a technically simple in vivo assay of long-term skin reg
2025-01-14 7 -
Real-Time RT-PCR for CTG Repeat-Containing Genes
Myotonic dystrophy (DM1) is a neuromuscular disorder caused by a CTGn expansion
2025-01-14 14 -
Isolation of Total RNA from Bacteria
The susceptibihty of RNA to degradation by exogenous and endogenous RNase activi
2025-01-14 9 -
The Analysis of Mitochondria and Mitochondrial DNA in Human Embryonic Stem Cells
As human embryonic stem cells (hESCs) undergo differentiation, they express gene
2025-01-14 9 -
支原体污染后的治疗和处理
第一种方法：使用泰乐菌素（tylosin），Sigma，120多块钱 泰乐菌素是一种兽用抗生素,常用在鸡、猪的食料辅料，可以防止支原体感染引起的支气管哮喘，至今
2025-01-14 7 -
Quantitating mRNAs with Relative and Competitive RT-PCR
Reverse transcription coupled with the polymerase chain reaction (RT-PCR), permi
2025-01-14 8 -
Reverse Transfected Cell Microarrays in Infectious Disease Research
Several human pathogenic viruses encode large genomes with often more than 100 g
2025-01-14 4 -
Copy Number Determination of Genetically-Modified Hematopoietic Stem Cells
Human gene transfer with gammaretroviral, murine leukemia virus (MLV) based vect
2025-01-14 7 -
High-Throughput Immunofluorescence Microscopy Using Yeast Spheroplast Cell-Based Microarrays
We have described a protocol for performing high-throughput immunofluorescence m
2025-01-14 4 -
CHIP引物设计四个注意事项详细点
1. 特异性确保引物与目标序列完全匹配：引物设计应基于目标DNA序列的精确信息，确保引物只与目标序列结合，而不与其他无关序列发生交叉反应。这可以通过使用生物信
2025-01-14 4
