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三羧酸循环(citric acid cycle)
由乙酰CoA和草酰乙酸缩合成有三个羧基的柠檬酸, 柠檬酸经一系列反应, 一再氧化脱羧, 经α酮戊二酸、 琥珀酸, 再降解成草酰乙酸。而参与这一循环的丙酮酸的三个
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细胞培养中的黑胶虫污染问题的处理
相关专题我们实验室最近一次的情况:我们前后从上海细胞中心买了三批细胞，细胞刚到的时候，观察均生长良好，密度适中，培养液清亮，也没有见小黑点，但是对细胞传代以后就
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The Cell Environment (including types of culture medium)
In general terms cultured cells require a sterile environment and a supply of nu
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杂交瘤细胞的制备方法
1） 瘤细胞培养（无特殊要求）骨髓瘤细胞呈悬浮或轻微附着形式生长，如附着性生长的细胞多时，用吸管轻轻吹打或轻轻叩击培养容器即可分离下来。通常要维持细胞于指数生长
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红细胞裂解的实验方法步骤
IntroductionPrior to using lymphoid tissue cell suspensions for flow cytometric
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Craniofacial Development of Avian and Rodent Embryos
In this overview, we examine approaches to the embryological development of the
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Analysis of Lymphocyte Development and Function Using the RAG-Deficient Blastocyst Complementation S
The RAG-deficient blastocyst complementation system (RBCS) represents a flexible
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去壁低渗法制备植物染色体标本实验
相关专题 一、实验目的： 1、掌屋植物染色体 标本的去壁低渗方法 2、了解中期染色体的形态结构 二、实验原理： 植物 细胞有很坚实的细胞壁，染色体很难像动物
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Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells
Cultured embryonic and adult skeletal muscle cells have a number of different us
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In Situ Detection of EpsteinBarr Virus and Phenotype Determination of EBV-Infected Cells
Epstein-Barr virus (EBV) establishes a lifelong infection of B cells. Consequent
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The Human Immunodeficiency Virus LTR-Promoter Region as a Reporter of Stress-Induced Gene Expression
Human immunodeficiency virus type-1 (HIV-1) replication and pro viral gene expre
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Single-Cell Enzymatic Dissociation of hESC Lines OxF1OxF4 and Culture in Feeder-Free Conditions
Experimental manipulation of hESCs has been hampered by their fragility and susc
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Cytotoxicity Assays Protocol
Cytotoxicity Assays ProtocolCell-mediated cytotoxicity was determined by using a
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粗面内质网(rough endoplasmic reticulum, RER)
多呈大的扁平膜囊状, 在电镜下观察排列极为整齐。它是核糖体和内质网共同构成的复合机能结构, 普遍存在于分泌蛋白质的细胞中, 越是分泌旺盛的细胞(如浆细胞)越多,
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Fluorescence Microscopy of Microtubules in Cultured Cells
Cytoplasmic microtubules are noncovalent polymers of the protein tubulin. In the
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