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Visualizing Signaling and Cell Movement During the Multicellular Stages of Dictyostelium Development
Time-lapse microscopy provides a powerful tool with which to study cell behavior
2025-01-14 6 -
多线染色体(polytene chromosome)
核内DNA多次复制产生的子染色体平行排列, 且体细胞内同源染色体配对, 紧密结合在一起, 从而阻止了染色体纤维进一步聚缩, 形成体积很大的由多条染色体组成的结构
2025-01-14 8 -
Immunofluorescence of Cultured Cells
The development and the extensive use of immunofluorescence microscopy on cultur
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DNA Damage Response and Mutagenesis in Mouse Embryonic Stem Cells
Mutation in embryonic stem (ES) cells can potentially compromise multiple cell l
2025-01-14 11 -
Tissue Procurement for Molecular Studies Using Laser-Assisted Microdissection
Properly collected and stored human specimens offer the unique opportunity to st
2025-01-14 5 -
Pharmacology of Antisense Therapeutic Agents: Cancer and Inflamination
Antisense oligonucleotides represent a new paradigm for drug discovery that hold
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Oligonucleotide-Directed Mutagenesis Using an Improved Phosphorothioate Approach
Oligonucleotide-directed in vitro mutagenesis is an established tool for the inv
2025-01-14 10 -
Generation of Nonviral Integration-Free Induced Pluripotent Stem Cells from Plucked Human Hair Folli
Human induced pluripotent stem cells (hiPSCs) provide a unique experimental reag
2025-01-14 5 -
Structural Studies of MAP Kinase Cascade Components
MAPK cascade components have been the subject of structural analysis, advancing
2025-01-14 5 -
RACE实验是什么？【分子生物学】
RACE实验的类型多样，包括经典RACE、Cap-switching RACE、环形RACE等。每种类型都有其独特的原理和应用场景，如经典RACE主要用于克隆c
2025-01-14 12 -
Preparation of DRV Liposomes
Dried reconstituted vesicles (DRV) are liposomes that are formulated under mild
2025-01-14 8 -
Procedures to Evaluate the Importance of Dietary Polyamines
Polyamines not only play vital physiological functions including modulating tran
2025-01-14 6 -
RACE实验提高扩增特异性和效率的新方法
一、优化引物设计选择合适的引物长度和GC含量：引物长度建议为23~28个核苷酸，GC含量为50%~70%。较高的GC比例和Tm值（65℃，若>70℃使
2025-01-14 9 -
RACE实验与基因表达分析
RACE实验与基因表达分析~首先还是从RACE实验的原理开始介绍，即cDNA末端快速扩增技术（Rapid Amplification of cDNA Ends）
2025-01-14 8 -
cDNA末端快速扩增技术（RACE）在基因克隆中的实践
一、RACE技术概述RACE技术，即Rapid Amplification of cDNA Ends，是一种基于PCR技术，从已知的部分cDNA序列出发，快速扩
2025-01-14 11
